ArticleThe EMBO journal1998
TAFII-independent activation mediated by human TBP in the presence of the positive cofactor PC4.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- BRD4: From molecular understanding to therapeutics development.Molecular cell · 2026Review
- ARF alters PAF1 complex integrity to selectively repress oncogenic transcription programs upon p53 loss.Molecular cell · 2024Article
- Rapid dynamics of general transcription factor TFIIB binding during preinitiation complex assembly revealed by single-molecule analysis.Genes & development · 2016Article
- Transcription of in vitro assembled chromatin templates in a highly purified RNA polymerase II system.Methods (San Diego, Calif.) · 2009Article
- Article
- CTCF interacts with and recruits the largest subunit of RNA polymerase II to CTCF target sites genome-wide.Molecular and cellular biology · 2007Article
- Transcriptional coactivator PC4, a chromatin-associated protein, induces chromatin condensation.Molecular and cellular biology · 2006Article
- Brd4 links chromatin targeting to HPV transcriptional silencing.Genes & development · 2006Article
- Transcriptional coactivator PC4 stimulates promoter escape and facilitates transcriptional synergy by GAL4-VP16.Molecular and cellular biology · 2004Article
- Human mediator enhances activator-facilitated recruitment of RNA polymerase II and promoter recognition by TATA-binding protein (TBP) independently of TBP-associated factors.Molecular and cellular biology · 2003Article
- Mutations in the histone fold domain of the TAF12 gene show synthetic lethality with the TAF1 gene lacking the TAF N-terminal domain (TAND) by different mechanisms from those in the SPT15 gene encoding the TATA box-binding protein (TBP).Nucleic acids research · 2003Article
- Multi-protein complexes in eukaryotic gene transcription.Plant molecular biology · 2002Review
- Molecular characterization of Saccharomyces cerevisiae TFIID.Molecular and cellular biology · 2002Article
- Requirement of TRAP/mediator for both activator-independent and activator-dependent transcription in conjunction with TFIID-associated TAF(II)s.Molecular and cellular biology · 2002Article
- Evidence that TAF-TATA box-binding protein interactions are required for activated transcription in mammalian cells.Molecular and cellular biology · 2002Article
- The regulatory role for the ERCC3 helicase of general transcription factor TFIIH during promoter escape in transcriptional activation.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Positive and negative TAF(II) functions that suggest a dynamic TFIID structure and elicit synergy with traps in activator-induced transcription.Molecular and cellular biology · 2001Article
- Region of yeast TAF 130 required for TFIID to associate with promoters.Molecular and cellular biology · 2001Article
- In vivo requirement of activator-specific binding targets of mediator.Molecular and cellular biology · 2000Article
- Robust mRNA transcription in chicken DT40 cells depleted of TAF(II)31 suggests both functional degeneracy and evolutionary divergence.Molecular and cellular biology · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
TFIID is a multiprotein complex comprised of the TATA-binding protein (TBP) and an array of TBP-associated factors (TAFIIs). Whereas TBP is sufficient for basal transcription in conjunction with other general transcription factors and RNA polymerase II, TAFIIs are additionally required for activator-dependent transcription in mammalian cell-free transcription systems. However, recent in vivo studies carried out in yeast suggest that TAFIIs are not globally required for activator function. The discrepancy between in vivo yeast studies and in vitro mammalian cell-free systems remains to be resolved. In this study, we describe a mammalian cell-free transcription system reconstituted with only recombinant proteins and epitope-tagged multiprotein complexes. Transcriptional activation can be recapitulated in this highly purified in vitro transcription system in the absence of TAFIIs. This TBP-mediated activation is not induced by human mediator, another transcriptional coactivator complex potentially implicated in activator response. In contrast, general transcription factors TFIIH and TFIIA play a significant role in TBP-mediated activation, which can be detected in vitro with Gal4 fusion proteins containing various transcriptional activation domains. Our data, therefore, suggest that TFIIH and TFIIA can mediate activator function in the absence of TAFIIs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.