ArticleApplied and environmental microbiology1998
A glutathione S-transferase with activity towards cis-1, 2-dichloroepoxyethane is involved in isoprene utilization by Rhodococcus sp. strain AD45.
Article in Applied and environmental microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 87 citations in OpenAlex.
- Structural and evolutionary insights into the isoprene monooxygenases.FEMS microbiology ecology · 2026Article
- Biodegradation of isoprene by soil Actinomycetota from coffee-tea integrated plantations in a tropical evergreen forest.Current research in microbial sciences · 2025Article
- Whole-cell studies of substrate and inhibitor specificity of isoprene monooxygenase and related enzymes.Environmental microbiology reports · 2023Article
- Analysis of Essential Isoprene Metabolic Pathway Proteins inApplied and environmental microbiology · 2023Article
- Article
- 'Omics-guided prediction of the pathway for metabolism of isoprene by Variovorax sp. WS11.Environmental microbiology · 2022Article
- Purification and Characterization of the Isoprene Monooxygenase fromApplied and environmental microbiology · 2022Article
- Genome Characterisation of an Isoprene-DegradingBiology · 2022Article
- Characterization of the GlutathioneMicrobiology spectrum · 2021Article
- Isoprene-degrading bacteria associated with the phyllosphere of Salix fragilis, a high isoprene-emitting willow of the Northern Hemisphere.Environmental microbiome · 2021Article
- Article
- Microbial metabolism of isoprene: a much-neglected climate-active gas.Microbiology (Reading, England) · 2020Review
- Molecular Ecology of Isoprene-Degrading Bacteria.Microorganisms · 2020Review
- Complete Genome of Isoprene DegradingMicroorganisms · 2020Article
- Isoprene Oxidation by the Gram-Negative Model bacteriumMicroorganisms · 2020Article
- Reductive metabolism of the important atmospheric gas isoprene by homoacetogens.The ISME journal · 2019Article
- Novel Isoprene-Degrading Proteobacteria From Soil and Leaves Identified by Cultivation and Metagenomics Analysis of Stable Isotope Probing Experiments.Frontiers in microbiology · 2019Article
- Poplar phyllosphere harbors disparate isoprene-degrading bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Gene probing reveals the widespread distribution, diversity and abundance of isoprene-degrading bacteria in the environment.Microbiome · 2018Article
- Heterologous Expression of Mycobacterium Alkene Monooxygenases in Gram-Positive and Gram-Negative Bacterial Hosts.Applied and environmental microbiology · 2018Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Rhodococcus sp. strain AD45 was isolated from an enrichment culture on isoprene (2-methyl-1,3-butadiene). Isoprene-grown cells of strain AD45 oxidized isoprene to 3,4-epoxy-3-methyl-1-butene, cis-1, 2-dichloroethene to cis-1,2-dichloroepoxyethane, and trans-1, 2-dichloroethene to trans-1,2-dichloroepoxyethane. Isoprene-grown cells also degraded cis-1,2-dichloroepoxyethane and trans-1, 2-dichloroepoxyethane. All organic chlorine was liberated as chloride during degradation of cis-1,2-dichloroepoxyethane. A glutathione (GSH)-dependent activity towards 3, 4-epoxy-3-methyl-1-butene, epoxypropane, cis-1,2-dichloroepoxyethane, and trans-1,2-dichloroepoxyethane was detected in cell extracts of cultures grown on isoprene and 3,4-epoxy-3-methyl-1-butene. The epoxide-degrading activity of strain AD45 was irreversibly lost upon incubation of cells with 1,2-epoxyhexane. A conjugate of GSH and 1, 2-epoxyhexane was detected in cell extracts of cells exposed to 1, 2-epoxyhexane, indicating that GSH is the physiological cofactor of the epoxide-transforming activity. The results indicate that a GSH S-transferase is involved in the metabolism of isoprene and that the enzyme can detoxify reactive epoxides produced by monooxygenation of chlorinated ethenes.
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