ArticleApplied and environmental microbiology1998
Expression and secretion of defined cutinase variants by Aspergillus awamori.
Article in Applied and environmental microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 38 citations in OpenAlex.
- Manufacturing of the highly active thermophile PETases PHL7 and PHL7mut3 using Escherichia coli.Microbial cell factories · 2024Article
- Biodegradation of plastic polymers by fungi: a brief review.Bioresources and bioprocessing · 2022Review
- Conditional Expression of the Small GTPase ArfA Impacts Secretion, Morphology, Growth, and Actin Ring Position inFrontiers in microbiology · 2018Article
- Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview.Microbial cell factories · 2008Article
- Cutinase-like enzyme from the yeast Cryptococcus sp. strain S-2 hydrolyzes polylactic acid and other biodegradable plastics.Applied and environmental microbiology · 2005Article
- Molecular characteristics of strains of the cameroon family, the major group of Mycobacterium tuberculosis in a country with a high prevalence of tuberculosis.Journal of clinical microbiology · 2004Article
- Modulation of Aspergillus awamori thaumatin secretion by modification of bipA gene expression.Applied and environmental microbiology · 2004Article
- Improvement of foreign-protein production in Aspergillus niger var. awamori by constitutive induction of the unfolded-protein response.Applied and environmental microbiology · 2003Article
- Calnexin overexpression increases manganese peroxidase production in Aspergillus niger.Applied and environmental microbiology · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several cutinase variants derived by molecular modelling and site-directed mutagenesis of a cutinase gene from Fusarium solani pisi are poorly secreted by Saccharomyces cerevisiae. The majority of these variants are successfully produced by the filamentous fungus Aspergillus awamori. However, the L51S and T179Y mutations caused reductions in the levels of extracellular production of two cutinase variants by A. awamori. Metabolic labelling studies were performed to analyze the bottleneck in enzyme production by the fungus in detail. These studies showed that because of the single L51S substitution, rapid extracellular degradation of cutinase occurred. The T179Y substitution did not result in enhanced sensitivity towards extracellular proteases. Presumably, the delay in the extracellular accumulation of this cutinase variant is caused by the enhanced hydrophobicity of the molecule. Overexpression of the A. awamori gene encoding the chaperone BiP in the cutinase-producing A. awamori strains had no significant effect on the secretion efficiency of the cutinases. A cutinase variant with the amino acid changes G28A, A85F, V184I, A185L, and L189F that was known to aggregate in the endoplasmic reticulum of S. cerevisiae, resulting in low extracellular protein levels, was successfully produced by A. awamori. An initial bottleneck in secretion occurred before or during translocation into the endoplasmic reticulum but was rapidly overcome by the fungus.
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