ArticleAntimicrobial agents and chemotherapy1998
Effect of growth rate on resistance of Candida albicans biofilms to antifungal agents.
Article in Antimicrobial agents and chemotherapy, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers, 1 of them a synthesis that pooled it.
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Who cites it
87 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Resistance profiles to antifungal agents in Candida albicans isolated from human oral cavities: systematic review and meta-analysis.Clinical oral investigations · 2022Pooled it
- Biofilm-Associated Candidiasis: Pathogenesis, Prevalence, Challenges and Therapeutic Options.Pharmaceuticals (Basel, Switzerland) · 2025Review
- The Effects of Carbonate on Candida albicans Filamentation, Biofilm Formation, and Antifungal Resistance.MicrobiologyOpen · 2024Article
- A ketogenic diet enhances fluconazole efficacy in murine models of systemic fungal infection.mBio · 2024Article
- Antifungal Susceptibility ofAntibiotics (Basel, Switzerland) · 2022Article
- Antifungal Exposure and Resistance Development: Defining Minimal Selective Antifungal Concentrations and Testing Methodologies.Frontiers in fungal biology · 2022Article
- Virulence Factors as Promoters of Chronic Vulvovaginal Candidosis: A Review.Mycopathologia · 2021Review
- Development of a novel denture care agent with highly active enzyme, arazyme.BMC oral health · 2021Article
- Sixty years of Amphotericin B: An Overview of the Main Antifungal Agent Used to Treat Invasive Fungal Infections.Infectious diseases and therapy · 2021Review
- Effects of two peroxide enzymatic denture cleaners on Candida albicans biofilms and denture surface.BMC oral health · 2020Article
- Inhibitory Effect of Morin AgainstFrontiers in microbiology · 2020Article
- Relationship between antifungal susceptibility profile and virulence factors in Candida albicans isolated from nail specimens.Revista da Sociedade Brasileira de Medicina Tropical · 2020Article
- Drug-mediated metabolic tipping between antibiotic resistant states in a mixed-species community.Nature ecology & evolution · 2018Article
- Chestnut Honey and Bacteriophage Application to ControlFrontiers in microbiology · 2018Article
- In Vitro Evaluation of the Inhibitory Activity of Thymoquinone in Combatting Candida albicans in Denture Stomatitis Prevention.International journal of environmental research and public health · 2017Article
- Candida glabrata Biofilms: How Far Have We Come?Journal of fungi (Basel, Switzerland) · 2017Review
- Candida Species Biofilms' Antifungal Resistance.Journal of fungi (Basel, Switzerland) · 2017Review
- Large-scale production and isolation of Candida biofilm extracellular matrix.Nature protocols · 2016Article
- Effect of Voriconazole on Candida tropicalis Biofilms: Relation with ERG Genes Expression.Mycopathologia · 2016Article
- Antimicrobial potential of two traditional herbometallic drugs against certain pathogenic microbial species.BMC complementary and alternative medicine · 2016Article
27 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A perfused biofilm fermentor, which allows growth-rate control of adherent microbial populations, was used to assess whether the susceptibility of Candida albicans biofilms to antifungal agents is dependent on growth rate. Biofilms were generated under conditions of glucose limitation and were perfused with drugs at a high concentration (20 times the MIC). Amphotericin B produced a greater reduction in the number of daughter cells in biofilm eluates than ketoconazole, fluconazole, or flucytosine. Similar decreases in daughter cell counts were observed when biofilms growing at three different rates were perfused with amphotericin B. In a separate series of experiments, intact biofilms, resuspended biofilm cells, and newly formed daughter cells were removed from the fermentor and were exposed to a lower concentration of amphotericin B for 1 h. The susceptibility profiles over a range of growth rates were then compared with those obtained for planktonic cells grown at the same rates under glucose limitation in a chemostat. Intact biofilms were resistant to amphotericin B at all growth rates tested, whereas planktonic cells were resistant only at low growth rates (</=0.13 h-1). Cells resuspended from biofilms were less resistant than intact biofilm populations but more resistant than daughter cells; the susceptibilities of both these cell types were largely independent of growth rate. Our findings indicate that the amphotericin B resistance of C. albicans biofilms is not simply due to a low growth rate but depends on some other feature of the biofilm mode of growth.
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