Evidence map›Paper›PMID 9687075›Full record

ArticlePlant molecular biology1998

Characterization of a cDNA encoding a novel plant poly(A) polymerase.

J Das Gupta, Q S Li, A B Thomson, A G Hunt

Abstract read
PubMed Publisher
In one paragraph

Article in Plant molecular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. A chloroplast cyclophilin functions in the assembly and maintenance of photosystem II in Arabidopsis thaliana.Proceedings of the National Academy of Sciences of the United States of America · 2007
    Article
  2. Article
  3. Review
  4. Plant mRNA 3'-end formation.Plant molecular biology · 1996
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

J Das GuptaDepartment of Agronomy, University of Kentucky, Lexington 40546-0091, USA.
Q S Li
A B Thomson
A G Hunt
University of Kentucky · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have isolated cDNA clones encoding a novel factor (PAP-I) that is a component of a multi-subunit poly(A) polymerase from pea seedlings. The encoded protein, when isolated from appropriately engineered Escherichia coli, was active as a poly(A) polymerase, either with an associated RNA binding cofactor (PAP-III) or with free poly(A) as an RNA substrate. The latter observation indicates that PAP-I is in fact a poly(A) polymerase. PAP-I bore a striking resemblance to an as yet uncharacterized cyanobacterial protein. This observation suggested a possible chloroplast localization for PAP-I. This hypothesis was tested and found to be substantiated; immunoblot analysis identified PAP-I in chloroplast but not nuclear extracts. Our results suggest that PAP-I is a component of the machinery that adds poly(A) to chloroplast RNAs.

Indexed as

Amino Acid SequenceBase SequenceChloroplastsDNA, ComplementaryDNA, PlantEscherichia coliMolecular Sequence DataPisum sativumPolynucleotide AdenylyltransferaseRecombinant Fusion ProteinsSequence Homology, Amino AcidDNA, ComplementaryDNA, PlantPolynucleotide AdenylyltransferaseRecombinant Fusion Proteins

Identifiers

PMID9687075
OpenAlexW149861733

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.