ArticleNucleic acids research1998
Assembly of MMTV promoter minichromosomes with positioned nucleosomes precludes NF1 access but not restriction enzyme cleavage.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- Pioneer factors and ATP-dependent chromatin remodeling factors interact dynamically: A new perspective: Multiple transcription factors can effect chromatin pioneer functions through dynamic interactions with ATP-dependent chromatin remodeling factors.BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Review
- Impact of chromatin structure on PR signaling: transition from local to global analysis.Molecular and cellular endocrinology · 2012Review
- Highly compacted chromatin formed in vitro reflects the dynamics of transcription activation in vivo.Molecular cell · 2010Article
- Nuclear factor 1 synergizes with progesterone receptor on the mouse mammary tumor virus promoter wrapped around a histone H3/H4 tetramer by facilitating access to the central hormone-responsive elements.The Journal of biological chemistry · 2010Article
- Erk signaling and chromatin remodeling in MMTV promoter activation by progestins.Nuclear receptor signaling · 2009Review
- Histone H1 subtypes differentially modulate chromatin condensation without preventing ATP-dependent remodeling by SWI/SNF or NURF.PloS one · 2009Article
- Two chromatin remodeling activities cooperate during activation of hormone responsive promoters.PLoS genetics · 2009Article
- A nucleosome positioned by alpha2/Mcm1 prevents Hap1 activator binding in vivo.Biochemical and biophysical research communications · 2007Article
- Ligand-specific dynamics of the progesterone receptor in living cells and during chromatin remodeling in vitro.Molecular and cellular biology · 2005Article
- Transcriptionally competent chromatin assembled with exogenous histones in a yeast whole cell extract.Nucleic acids research · 2004Article
- Histone H1 enhances synergistic activation of the MMTV promoter in chromatin.The EMBO journal · 2003Article
- ATP-dependent mobilization of the glucocorticoid receptor during chromatin remodeling.Molecular and cellular biology · 2002Article
- Hormone-induced nucleosome positioning in the MMTV promoter is reversible.The EMBO journal · 2001Article
- Hormone activation induces nucleosome positioning in vivo.The EMBO journal · 2000Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To generate long arrays of nucleosomes within a topologically defined chromatin domain we have assembled minichromosomes on negatively supercoiled plasmid DNA with extracts from Drosophila preblastoderm embryos. These minichromosomes are dynamic substrates for energy-dependent nucleosome remodeling machines that facilitate the binding of various transcription factors but do not exhibit nucleosome positioning. In contrast, if such minichromosomes include the mouse mammary tumour virus (MMTV) promoter we find it wrapped around a nucleosome with similar translational and rotational position as in vivo . This structure precluded binding of NF1 to its cognate site at -75/-65 at salt concentrations between 60 and 120 mM, even in the presence of ATP, which rendered the NF1 site accessible to the restriction enzyme Hin fI. However, insertion of 30 bp just upstream of the NF1 site, which moves the site to the linker DNA, allowed ATP-dependent binding of NF1 to a fraction of the minichromosomes, even in the presence ofstoichiometric amounts of histone H1. The minichromosomes assembled in the Drosophila embryo extract reproduce important features of the native MMTV promoter chromatin and reveal differences in the ability of transcription factors and restriction enzymes to access their binding sites in positioned nucleosomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.