ArticleNucleic acids research1998
Characterization of Schizosaccharomyces pombe Rad2 protein, a FEN-1 homolog.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- PbrFEN-1 positively regulates pear pollen tube growth and is involved in the self-incompatibility response.Plant reproduction · 2026Article
- Gene cloning and characterization of Tk1281, a flap endonuclease 1 from Thermococcus kodakarensis.Folia microbiologica · 2020Article
- Ctp1-dependent clipping and resection of DNA double-strand breaks by Mre11 endonuclease complex are not genetically separable.Nucleic acids research · 2016Article
- Functional analyses of human DNA repair proteins important for aging and genomic stability using yeast genetics.DNA repair · 2012Review
- Rice exonuclease-1 homologue, OsEXO1, that interacts with DNA polymerase lambda and RPA subunit proteins, is involved in cell proliferation.Plant molecular biology · 2008Article
- Fission yeast Dna2 is required for generation of the telomeric single-strand overhang.Molecular and cellular biology · 2004Article
- The Mus81 solution to resolution: generating meiotic crossovers without Holliday junctions.Genes & development · 2004Review
- DmGEN, a novel RAD2 family endo-exonuclease from Drosophila melanogaster.Nucleic acids research · 2004Article
- OsSEND-1: a new RAD2 nuclease family member in higher plants.Plant molecular biology · 2003Article
- Fission yeast Rad50 stimulates sister chromatid recombination and links cohesion with repair.The EMBO journal · 2001Article
- The nature of the 5'-terminus is a major determinant for DNA processing by Schizosaccharomyces pombe Rad2p, a FEN-1 family nuclease.Nucleic acids research · 2000Article
- Plant homologue of flap endonuclease-1: molecular cloning, characterization, and evidence of expression in meristematic tissues.Plant molecular biology · 2000Article
- Saccharomyces cerevisiae RNase H(35) functions in RNA primer removal during lagging-strand DNA synthesis, most efficiently in cooperation with Rad27 nuclease.Molecular and cellular biology · 1999Article
- A Uve1p-mediated mismatch repair pathway in Schizosaccharomyces pombe.Molecular and cellular biology · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
FEN-1 proteins are a family of nucleases essential for lagging strand DNA synthesis. A gene with sequence similarity to FEN-1 protein-encoding genes, rad2 +, has been identified in Schizosaccharomyces pombe . We report the overexpression, purification, and character-ization of the putative S.pombe FEN-1 homolog, Rad2p. A GST-Rad2p fusion protein was over-expressed in Saccharomyces cerevisiae and purified to near homogeneity by GST affinity chromatography. Although Rad2p had been previously classified as a putative FEN-1 protein based on amino acid homology, there has been no biochemical evidence demonstrating flap endonuclease activity. DNA cleavage analysis of several different oligodeoxynucleotide structuresindicates that GST-Rad2p possesses both 5'-flap endonuclease and 5'-->3' double-stranded DNA exo-nuclease activities. GST-Rad2p incises a 5'-flap and a 5'-pseudo-Y structure one base 3' of the branch point in the duplex region and also degrades double-stranded DNA. This is the first report on the biochemical characterization of S.pombe Rad2p. The potential roles of Rad2p in DNA excision repair and other nucleic acid reactions are discussed.
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