ArticleAmerican journal of human genetics1998
From amplification to gene in thyroid cancer: a high-resolution mapped bacterial-artificial-chromosome resource for cancer chromosome aberrations guides gene discovery after comparative genome hybridization.
Article in American journal of human genetics, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
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- Genetic copy number alterations and IL-13 expression differences in papillary thyroid cancers and benign nodules.Endocrine · 2009Article
- Protein kinase C epsilon: an oncogene and emerging tumor biomarker.Molecular cancer · 2009Review
- Methods for high throughput validation of amplified fragment pools of BAC DNA for constructing high resolution CGH arrays.BMC genomics · 2004Article
- Chromosome 2 (2p16) abnormalities in Carney complex tumours.Journal of medical genetics · 2003Article
- Conditional rescue of protein kinase C epsilon regulates ethanol preference and hypnotic sensitivity in adult mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Molecular genetics and structural genomics of the human protein kinase C gene module.Genome biology · 2002Article
- Human genome anatomy: BACs integrating the genetic and cytogenetic maps for bridging genome and biomedicine.Genome research · 1999Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
Chromosome rearrangements associated with neoplasms provide a rich resource for definition of the pathways of tumorigenesis. The power of comparative genome hybridization (CGH) to identify novel genes depends on the existence of suitable markers, which are lacking throughout most of the genome. We now report a general approach that translates CGH data into higher-resolution genomic-clone data that are then used to define the genes located in aneuploid regions. We used CGH to study 33 thyroid-tumor DNAs and two tumor-cell-line DNAs. The results revealed amplifications of chromosome band 2p21, with less-intense amplification on 2p13, 19q13.1, and 1p36 and with least-intense amplification on 1p34, 1q42, 5q31, 5q33-34, 9q32-34, and 14q32. To define the 2p21 region amplified, a dense array of 373 FISH-mapped chromosome 2 bacterial artificial chromosomes (BACs) was constructed, and 87 of these were hybridized to a tumor-cell line. Four BACs carried genomic DNA that was amplified in these cells. The maximum amplified region was narrowed to 3-6 Mb by multicolor FISH with the flanking BACs, and the minimum amplicon size was defined by a contig of 420 kb. Sequence analysis of the amplified BAC 1D9 revealed a fragment of the gene, encoding protein kinase C epsilon (PKCepsilon), that was then shown to be amplified and rearranged in tumor cells. In summary, CGH combined with a dense mapped resource of BACs and large-scale sequencing has led directly to the definition of PKCepsilon as a previously unmapped candidate gene involved in thyroid tumorigenesis.
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