ArticleThe Journal of physiology1998
Non-genomic action of the mineralocorticoid aldosterone on cytosolic sodium in cultured kidney cells.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 54 citations in OpenAlex.
- ChIP happens: from biochemical origins to the modern omics toolbox for understanding steroid hormone receptors.The Biochemical journal · 2026Review
- Aldosterone: From Essential Tubular Regulator to Pathological Driver-Physiology, Disease, and Therapeutic Advances.International journal of molecular sciences · 2025Review
- Natural cardenolides suppress coronaviral replication by downregulating JAK1 via a NaBiochemical pharmacology · 2020Article
- Genomic and rapid effects of aldosterone: what we know and do not know thus far.Heart failure reviews · 2017Review
- Rapid Action of Aldosterone on Protein Levels of Sodium-Hydrogen Exchangers and Protein Kinase C Beta Isoforms in Rat Kidney.International journal of endocrinology · 2017Article
- Role of Nongenomic Signaling Pathways Activated by Aldosterone During Cardiac Reperfusion Injury.Molecular endocrinology (Baltimore, Md.) · 2015Article
- G protein-coupled receptors: extranuclear mediators for the non-genomic actions of steroids.International journal of molecular sciences · 2014Review
- The multifaceted mineralocorticoid receptor.Comprehensive Physiology · 2014Review
- Expression of mineralocorticoid and glucocorticoid receptors in preautonomic neurons of the rat paraventricular nucleus.American journal of physiology. Regulatory, integrative and comparative physiology · 2014Article
- Aldosterone stimulates elastogenesis in cardiac fibroblasts via mineralocorticoid receptor-independent action involving the consecutive activation of Galpha13, c-Src, the insulin-like growth factor-I receptor, and phosphatidylinositol 3-kinase/Akt.The Journal of biological chemistry · 2009Article
- Actions of aldosterone in the cardiovascular system: the good, the bad, and the ugly?Pflugers Archiv : European journal of physiology · 2009Article
- Aldosterone induces elastin production in cardiac fibroblasts through activation of insulin-like growth factor-I receptors in a mineralocorticoid receptor-independent manner.The American journal of pathology · 2007Article
- Metalloregulation of yeast membrane steroid receptor homologs.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Review
- Rapid and non-genomic reduction of intracellular [Ca(2+)] induced by aldosterone in human bronchial epithelium.The Journal of physiology · 2001Article
- Inhibition of Na+-H+ exchange impairs receptor-mediated albumin endocytosis in renal proximal tubule-derived epithelial cells from opossum.The Journal of physiology · 1999Article
- Effect of aldosterone on the regulation of the volume of principal cells of rat cortical collecting duct epithelium in early postnatal development.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sectionsArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The mineralocorticoid aldosterone is essential for the regulation of electrolyte homeostasis, extracellular volume and blood pressure. As a steroid hormone the classical way of action is genomic. Previously we reported a non-genomic action of aldosterone on cytosolic Ca2+ and pH in renal epithelial (MDCK) cells. In parallel, aldosterone induces Zn2+-sensitive cytosolic acidification when extracellular Na+ is absent. 2. We now show that aldosterone (EC50, 7 x 10-11 mol l-1) induces a non-genomic increase in cytosolic sodium in MDCK cells. The membrane-impermeable aldosterone-bovine serum albumin (BSA) conjugate exerted the same effect. The effect of aldosterone was completely abolished by inhibition of Na+-H+ exchange with ethyl-isopropanol amiloride (EIPA). Aldosterone-induced Na+ influx exceeded H+ efflux more than 10-fold. 3. Omission of extracellular Ca2+, inhibition of protein kinase C or pretreatment with pertussis toxin reduced the effect of aldosterone significantly. Zn2+ (IC50, 3.3 x 10-6 mol l-1), but not ouabain, abolished the increase in Na+ almost completely. 4. The aldosterone-induced increase in cytosolic sodium was accompanied by an EIPA- and Zn2+-sensitive cell swelling. 5. Thus, physiological concentrations of aldosterone induce a non-genomic increase in cytosolic sodium concentration by activation of Na+-H+ exchange. Aldosterone exerts its effect, at least in part, at the plasma membrane via interaction with a G-protein-coupled mechanism. 6. The simultaneous activation of the acidification mechanism and Na+-H+ exchange by aldosterone allows a dramatic sodium influx without excessive changes in cytosolic pH and leads to changes in cell volume.
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