ArticleThe Journal of physiology1998
ATP-evoked increases in [Ca2+]i and peptide release from rat isolated neurohypophysial terminals via a P2X2 purinoceptor.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 87 citations in OpenAlex.
- Cell Type-Specific Expression of Purinergic P2X Receptors in the Hypothalamus.International journal of molecular sciences · 2025Review
- AVPVThe Journal of reproduction and development · 2024Article
- P2X2 Receptor Expression and Function Is Upregulated in the Rat Supraoptic Nucleus Stimulated Through Refeeding After Fasting.Frontiers in cellular neuroscience · 2019Article
- Effects of calcium and sodium on ATP-induced vasopressin release from rat isolated neurohypophysial terminals.Journal of neuroendocrinology · 2018Article
- Purinergic receptor types in the hypothalamic-neurohypophysial system.Journal of neuroendocrinology · 2018Review
- Extracellular ATP and other nucleotides-ubiquitous triggers of intercellular messenger release.Purinergic signalling · 2016Review
- Purinergic signaling pathways in endocrine system.Autonomic neuroscience : basic & clinical · 2015Review
- Nutritional state-dependent ghrelin activation of vasopressin neurons via retrograde trans-neuronal-glial stimulation of excitatory GABA circuits.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- P2X7 receptors in neurohypophysial terminals: evidence for their role in arginine-vasopressin secretion.Journal of cellular physiology · 2014Article
- Purinergic signalling in endocrine organs.Purinergic signalling · 2014Review
- Potentiation of inhibitory synaptic transmission by extracellular ATP in rat suprachiasmatic nuclei.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- P2X receptor channels in endocrine glands.Wiley interdisciplinary reviews. Membrane transport and signaling · 2013Article
- P2X purinergic receptor knockout mice reveal endogenous ATP modulation of both vasopressin and oxytocin release from the intact neurohypophysis.Journal of neuroendocrinology · 2012Article
- Adenosine trisphosphate appears to act via different receptors in terminals versus somata of the hypothalamic neurohypophysial system.Journal of neuroendocrinology · 2012Article
- Bench-to-bedside review: Vasopressin in the management of septic shock.Critical care (London, England) · 2011Review
- Ion channels and signaling in the pituitary gland.Endocrine reviews · 2010Review
- Signaling by purinergic receptors and channels in the pituitary gland.Molecular and cellular endocrinology · 2010Review
- Purinergic regulation of hypothalamopituitary functions.Trends in endocrinology and metabolism: TEM · 2009Review
- Sustained stimulation of vasopressin and oxytocin release by ATP and phenylephrine requires recruitment of desensitization-resistant P2X purinergic receptors.American journal of physiology. Regulatory, integrative and comparative physiology · 2009Article
- P2X receptors are differentially expressed on vasopressin- and oxytocin-containing neurons in the supraoptic and paraventricular nuclei of rat hypothalamus.Histochemistry and cell biology · 2009Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
1. The effect of externally applied ATP on cytosolic free Ca2+ concentration ([Ca2+]i) was tested in single isolated rat neurohypophysial nerve terminals by fura-2 imaging. The release of vasopressin (AVP) and oxytocin (OT) upon ATP stimulation was also studied from a population of terminals using specific radioimmunoassays. 2. ATP evoked a sustained [Ca2+]i increase, which was dose dependent in the 1-100 microM range (EC50 = 4.8 microM). This effect was observed in only approximately 40 % of the terminals. 3. Interestingly, ATP, in the same range (EC50 = 8.6 microM), evoked AVP, but no significant OT, release from these terminals. 4. Both the [Ca2+]i increase and AVP release induced by ATP were highly and reversibly inhibited by suramin, suggesting the involvement of a P2 purinergic receptor in the ATP-induced responses. Pyridoxal-5-phosphate-6-azophenyl-2',4'-disulphonic acid (PPADS), another P2 purinergic receptor antagonist, strongly reduced the ATP-induced [Ca2+]i response. 5. To further characterize the receptor, different agonists were tested, with the following efficacy: ATP = 2-methylthio-ATP > ATP-gamma-S > alpha, beta-methylene-ATP > ADP. The compounds adenosine, AMP, beta, gamma-methylene-ATP and UTP were ineffective. 6. The ATP-dependent [Ca2+]i increase was dependent on extracellular Ca2+ concentration ([Ca2+]o). Fluorescence-quenching experiments with Mn2+ showed that externally applied ATP triggered a Mn2+ influx. The ATP-induced [Ca2+]i increase and AVP release were independent of and additive to a K+-induced response, in addition to being insensitive to Cd2+. The ATP-induced [Ca2+]i increase was strongly reduced in the presence of Gd3+. These results suggest that the observed [Ca2+]i increases were elicited by Ca2+ entry through a P2X channel receptor rather than via a voltage-dependent Ca2+ channel. 7. We propose that ATP, co-released with neuropeptides, could act as a paracrine-autocrine messenger, stimulating, via Ca2+ entry through a P2X2 receptor, the secretion of AVP, in particular, from neurohypophysial nerve terminals.
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