ArticleThe Journal of physiology1998
Osmosensitive C1- currents and their relevance to regulatory volume decrease in human intestinal T84 cells: outwardly vs. inwardly rectifying currents.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 66 citations in OpenAlex.
- LRRC8A-containing chloride channel is crucial for cell volume recovery and survival under hypertonic conditions.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Cell Death Induction and Protection by Activation of Ubiquitously Expressed Anion/Cation Channels. Part 1: Roles of VSOR/VRAC in Cell Volume Regulation, Release of Double-Edged Signals and Apoptotic/Necrotic Cell Death.Frontiers in cell and developmental biology · 2020Review
- SWELL1 promotes cell growth and metastasis of hepatocellular carcinoma in vitro and in vivo.EBioMedicine · 2019Article
- The LRRC8-mediated volume-regulated anion channel is altered in glaucoma.Scientific reports · 2019Article
- Research and progress on ClC‑2 (Review).Molecular medicine reports · 2017Review
- Mechanosensitivity of wild-type and G551D cystic fibrosis transmembrane conductance regulator (CFTR) controls regulatory volume decrease in simple epithelia.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- Ionic currents of human trabecular meshwork cells from control and glaucoma subjects.The Journal of membrane biology · 2013Article
- Relative contribution of chloride channels and transporters to regulatory volume decrease in human glioma cells.American journal of physiology. Cell physiology · 2005Article
- Antagonistic regulation of swelling-activated Cl- current in rabbit ventricle by Src and EGFR protein tyrosine kinases.American journal of physiology. Heart and circulatory physiology · 2005Article
- Article
- Expression of voltage-gated chloride channels in human glioma cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Effect of an N-terminus deletion on voltage-dependent gating of the ClC-2 chloride channel.The Journal of physiology · 2002Article
- Small bowel review: normal physiology part 1.Digestive diseases and sciences · 2001Review
- Volume-activated chloride currents contribute to the resting conductance and invasive migration of human glioma cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Defective regulatory volume decrease in human cystic fibrosis tracheal cells because of altered regulation of intermediate conductance Ca2+-dependent potassium channels.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Pharmacological characterization of volume-sensitive, taurine permeable anion channels in rat supraoptic glial cells.British journal of pharmacology · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The swelling-activated outwardly rectifying Cl- current (ICl(swell)) recorded in T84 human intestinal cells was completely blocked by 10 microM tamoxifen, while 300 microM Cd2+ had no effect. 2. A ClC-2-like, inwardly rectifying Cl- current was activated after strong hyperpolarization in T84 cells. This current was completely inhibited by 300 microM Cd2+, unaffected by 10 microM tamoxifen, and its magnitude increased slightly in response to cell swelling under hyposmotic conditions. However, the swelling-dependent modulation occurred only after prior activation by hyperpolarizing voltages. 3. T84 cells behaved initially close to perfect osmometers in response to changes in external osmolalities between +20 and -30 %. The cells underwent full regulatory volume decrease (RVD) within 16 min when exposed to 30 or 10 % hyposmotic shocks. 4. Pharmacological tools were used to determine the anionic pathway(s) involved in RVD in T84 cells. Tamoxifen (10 microM), 1,9-dideoxyforskolin (DDFSK; 100 microM) and 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS; 100 microM) blocked RVD while 300 microM Cd2+ had no effect upon RVD following a 30 % hyposmotic shock. The RVD response was similarly unaffected by Cd2+ when cells were exposed to a smaller (10 %) hyposmotic shock. 5. In conclusion, these data show that the anionic pathway primarily activated by cell swelling and relevant to RVD in T84 cells is the tamoxifen-, DDFSK- and DIDS-sensitive ICl(swell) and not the hyperpolarization-activated, Cd2+-sensitive Cl- current associated with the ClC-2 Cl- channel.
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