ArticleThe Journal of cell biology1998
Pex20p of the yeast Yarrowia lipolytica is required for the oligomerization of thiolase in the cytosol and for its targeting to the peroxisome.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- Building peroxisomes: perspectives on plant peroxins.Biochemical Society transactions · 2026Review
- Identifying and characterizing a missing peroxin-PEX8-in Arabidopsis thaliana.The Plant cell · 2025Article
- Fusarium verticillioides Pex7/20 mediates peroxisomal PTS2 pathway import, pathogenicity, and fumonisin B1 biosynthesis.Applied microbiology and biotechnology · 2022Article
- The autophagic degradation of cytosolic pools of peroxisomal proteins by a new selective pathway.Autophagy · 2020Article
- Mechanisms by which PE21, an extract from the white willowOncotarget · 2019Article
- Current Advances in Protein Import into Peroxisomes.The protein journal · 2019Review
- Mechanisms through which lithocholic acid delays yeast chronological aging under caloric restriction conditions.Oncotarget · 2018Article
- Hansenula polymorpha Aat2p is targeted to peroxisomes via a novel Pex20p-dependent pathway.FEBS letters · 2018Article
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- A molecular genetic toolbox forBiotechnology for biofuels · 2017Article
- Mechanistic insights into PTS2-mediated peroxisomal protein import: the co-receptor PEX5L drastically increases the interaction strength between the cargo protein and the receptor PEX7.The Journal of biological chemistry · 2015Article
- The similarity between N-terminal targeting signals for protein import into different organelles and its evolutionary relevance.Frontiers in physiology · 2015Review
- Lithocholic bile acid accumulated in yeast mitochondria orchestrates a development of an anti-aging cellular pattern by causing age-related changes in cellular proteome.Cell cycle (Georgetown, Tex.) · 2015Article
- The unique degradation pathway of the PTS2 receptor, Pex7, is dependent on the PTS receptor/coreceptor, Pex5 and Pex20.Molecular biology of the cell · 2014Article
- Mechanism of liponecrosis, a distinct mode of programmed cell death.Cell cycle (Georgetown, Tex.) · 2014Article
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- Global analysis of condition-specific subcellular protein distribution and abundance.Molecular & cellular proteomics : MCP · 2013Article
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- Lipid droplets and peroxisomes: key players in cellular lipid homeostasis or a matter of fat--store 'em up or burn 'em down.Genetics · 2013Review
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pex mutants are defective in peroxisome assembly. In the pex20-1 mutant strain of the yeast Yarrowia lipolytica, the peroxisomal matrix protein thiolase is mislocalized exclusively to the cytosol, whereas the import of other peroxisomal proteins is unaffected. The PEX20 gene was isolated by functional complementation of the pex20-1 strain and encodes a protein, Pex20p, of 424 amino acids (47,274 D). Despite its role in the peroxisomal import of thiolase, which is targeted by an amino-terminal peroxisomal targeting signal-2 (PTS2), Pex20p does not exhibit homology to Pex7p, which acts as the PTS2 receptor. Pex20p is mostly cytosolic, whereas 4-8% is associated with high-speed (200,000 g) pelletable peroxisomes. In the wild-type strain, all newly synthesized thiolase is associated with Pex20p in a heterotetrameric complex composed of two polypeptide chains of each protein. This association is independent of PTS2. Pex20p is required for both the oligomerization of thiolase in the cytosol and its targeting to the peroxisome. Our data suggest that monomeric Pex20p binds newly synthesized monomeric thiolase in the cytosol and promotes the formation of a heterotetrameric complex of these two proteins, which could further bind to the peroxisomal membrane. Translocation of the thiolase homodimer into the peroxisomal matrix would release Pex20p monomers back to the cytosol, thereby permitting a new cycle of binding-oligomerization-targeting-release for Pex20p and thiolase.
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