ArticleGenes & development1998
Genetic analysis of the SR protein ASF/SF2: interchangeability of RS domains and negative control of splicing.
Article in Genes & development, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed, 98 citations in OpenAlex.
- Article
- Pathogenic variants that alter protein code often disrupt splicing.Nature genetics · 2017Article
- An Ultraconserved Element (UCE) controls homeostatic splicing of ARGLU1 mRNA.Nucleic acids research · 2017Article
- Article
- Regulation of alternative splicing of tau exon 10 by 9G8 and Dyrk1A.Neurobiology of aging · 2012Article
- RNAP II CTD phosphorylated on threonine-4 is required for histone mRNA 3' end processing.Science (New York, N.Y.) · 2011Article
- Stress-responsive maturation of Clk1/4 pre-mRNAs promotes phosphorylation of SR splicing factor.The Journal of cell biology · 2011Article
- Using positional distribution to identify splicing elements and predict pre-mRNA processing defects in human genes.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Human PRP4 kinase is required for stable tri-snRNP association during spliceosomal B complex formation.Nature structural & molecular biology · 2010Article
- SR proteins in vertical integration of gene expression from transcription to RNA processing to translation.Molecular cell · 2009Review
- Increased dosage of Dyrk1A alters alternative splicing factor (ASF)-regulated alternative splicing of tau in Down syndrome.The Journal of biological chemistry · 2008Article
- The splicing factor SC35 has an active role in transcriptional elongation.Nature structural & molecular biology · 2008Article
- Feline immunodeficiency virus OrfA alters gene expression of splicing factors and proteasome-ubiquitination proteins.Virology · 2008Article
- Processive phosphorylation: mechanism and biological importance.Cellular signalling · 2007Review
- Deletion of the N-terminus of SF2/ASF permits RS-domain-independent pre-mRNA splicing.PloS one · 2007Article
- Axis inhibition protein 2 (AXIN2) polymorphisms may be a risk factor for selective tooth agenesis.Journal of human genetics · 2006Article
- Loss of splicing factor ASF/SF2 induces G2 cell cycle arrest and apoptosis, but inhibits internucleosomal DNA fragmentation.Genes & development · 2005Article
- Multiple properties of the splicing repressor SRp38 distinguish it from typical SR proteins.Molecular and cellular biology · 2005Article
- Global analysis of positive and negative pre-mRNA splicing regulators in Drosophila.Genes & development · 2005Article
- Human immunodeficiency virus type 2 Gag interacts specifically with PRP4, a serine-threonine kinase, and inhibits phosphorylation of splicing factor SF2.Journal of virology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The SR proteins constitute a family of splicing factors, highly conserved in metazoans, that contain one or two amino-terminal RNA-binding domains (RBDs) and a region enriched in arginine/serine repeats (RS domain) at the carboxyl terminus. Previous studies have shown that SR proteins possess distinct RNA-binding specificities that likely contribute to their unique functions, but it is unclear whether RS domains have specific roles in vivo. Here, we used a genetic system developed in the chicken B cell line DT40 to address this question. Expression of chimeric proteins generated by fusion of the RS domains of heterologous SR proteins, or a human TRA-2 protein, with the RBDs of ASF/SF2 allowed cell growth following genetic inactivation of endogenous ASF/SF2, indicating that RS domains are interchangeable for all functions required to maintain cell viability. However, a chimera containing the RS domain from a related splicing factor, U2AF65, could not rescue viability and was inactive in in vitro splicing assays, suggesting that this domain performs a distinct function. We also used the DT40 system to show that depletion of ASF/SF2 affects splicing of specific transcripts in vivo. Although splicing of several simple constitutive introns was not significantly affected, the alternative splicing patterns of two model pre-mRNAs switched in a manner consistent with predictions from previous studies. Unexpectedly, ASF/SF2 depletion resulted in a substantial increase in splicing of an HIV-1 tat pre-mRNA substrate, indicating that ASF/SF2 can repress tat splicing in vivo. These results provide the first demonstration that an SR protein can influence splicing of specific pre-mRNAs in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.