ArticlePlant molecular biology1998
An as-1-like motif controls the level of expression of the gene for the pathogenesis-related protein 1a from tobacco.
Article in Plant molecular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed.
- Mediator subunit MED19 modulates elf18-inducedPlant signaling & behavior · 2026Article
- Genome-wide identification and characterization of SET domain family genes in Neopyropia yezoensis.BMC genomics · 2026Article
- Nanomaterial inactivates environmental virus and enhances plant immunity for controlling tobacco mosaic virus disease.Nature communications · 2024Article
- The expression of the NPR1-dependent defense response pathway genes in Persea americana (Mill.) following infection with Phytophthora cinnamomi.BMC plant biology · 2023Article
- Synthetic dual hormone-responsive promoters enable engineering of plants with broad-spectrum resistance.Plant communications · 2023Article
- Transcriptome analysis reveals pathogenesis-related gene 1 pathway against salicylic acid treatment in grapevine (Frontiers in genetics · 2022Article
- Biofoundry-assisted expression and characterization of plant proteins.Synthetic biology (Oxford, England) · 2021Article
- Dissecting the Role of Promoters of Pathogen-sensitive Genes in Plant Defense.Current genomics · 2020Review
- Plant Soft Rot Development and Regulation from the Viewpoint of Transcriptomic Profiling.Plants (Basel, Switzerland) · 2020Article
- The regulation ofPlant direct · 2019Article
- The NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1) and Related Family: Mechanistic Insights in Plant Disease Resistance.Frontiers in plant science · 2019Review
- Identification and expression analysis of genes with pathogen-inducible cis-regulatory elements in the promoter regions in Oryza sativa.Rice (New York, N.Y.) · 2018Article
- Arabidopsis WRKY50 and TGA Transcription Factors Synergistically Activate Expression ofFrontiers in plant science · 2018Article
- Global nucleosome positioning regulates salicylic acid mediated transcription in Arabidopsis thaliana.BMC plant biology · 2015Article
- The Arabidopsis NIMIN proteins affect NPR1 differentially.Frontiers in plant science · 2013Article
- To close or not to close: plasmodesmata in defense.Plant signaling & behavior · 2012Article
- NONEXPRESSOR OF PATHOGENESIS-RELATED PROTEINS1 (NPR1) and some NPR1-related proteins are sensitive to salicylic acid.Molecular plant pathology · 2011Article
- Tobacco Transcription Factor NtWRKY12 Interacts with TGA2.2 in vitro and in vivo.Frontiers in plant science · 2011Article
- In vivo characterization of plant promoter element interaction using synthetic promoters.Transgenic research · 2008Article
- A Novel WRKY transcription factor is required for induction of PR-1a gene expression by salicylic acid and bacterial elicitors.Plant physiology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pathogenesis-related proteins of group 1 (PR-1) are strongly induced in plants by pathogen attack, exposure of the plants to (acetyl)salicylic acid (ASA, SA), and by developmental cues. Functional analysis of the PR-1a promoter identified a region of 139 bp (from -691 to -553) mediating expression of the GUS reporter gene in response to ASA. Inspection of this region revealed two TGACG elements reminiscent of activation sequence-1 (as-1). Recently, as-1 has been reported to be responsive to SA in the context of the CaMV 35S RNA promoter. To address the question of whether the as-1-like sequence may be of functional significance for the expression of the PR-1a gene, gel shift assays were performed with TGA1a, a protein been shown to interact with as-1 in vitro. TGA1a was found to bind to the PR-1a as-1-like sequence with similar specificity and affinity as to as-1. Furthermore, mutations were introduced in the as-1-like sequence in the context of the inducible 906 bp PR-1a promoter which are impaired in binding TGA1a in vitro. Significantly reduced levels of GUS reporter gene activity were obtained with the mutant promoter regions as compared to the wild-type PR-1a promoter in response to all stimuli in transgenic tobacco plants. Yet, mutation of the as-1-like sequence did not abolish induction of reporter gene expression. Taken together, these results suggest that the level of expression of the tobacco PR-1a gene is controlled by an as-1-like sequence motif in the PR-1a upstream region, possibly interacting with a factor related to TGA1a.
Indexed as
Identifiers
9678582What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.