ArticlePlant molecular biology1998
Molecular cloning of a novel Ca2+-binding protein that is induced by NaCl stress.
Article in Plant molecular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 54 citations in OpenAlex.
- Walnut phosphatase 2A proteins interact with basic leucine zipper protein JrVIP1 to regulate osmotic stress responseForestry research · 2024Article
- Genomic Analysis of Soybean PP2A-BFrontiers in plant science · 2021Article
- The Molecular Basis of Kale Domestication: Transcriptional Profiling of Developing Leaves Provides New Insights Into the Evolution of aFrontiers in plant science · 2021Article
- The Arabidopsis NAC transcription factor ANAC096 cooperates with bZIP-type transcription factors in dehydration and osmotic stress responses.The Plant cell · 2013Article
- Identification of drought tolerant progenies in tea by gene expression analysis.Functional & integrative genomics · 2012Article
- A vacuolar β-glucosidase homolog that possesses glucose-conjugated abscisic acid hydrolyzing activity plays an important role in osmotic stress responses in Arabidopsis.The Plant cell · 2012Article
- Molecular and genetic evidence for the key role of AtCaM3 in heat-shock signal transduction in Arabidopsis.Plant physiology · 2009Article
- Calcium in plants.Annals of botany · 2003Review
- Analysis of EF-hand-containing proteins in Arabidopsis.Genome biology · 2002Article
- Calmodulins and calcineurin B-like proteins: calcium sensors for specific signal response coupling in plants.The Plant cell · 2002Review
- The ACA4 gene of Arabidopsis encodes a vacuolar membrane calcium pump that improves salt tolerance in yeast.Plant physiology · 2000Article
- A rapid induction by elicitors of the mRNA encoding CCD-1, a 14kDa Ca2+ -binding protein in wheat cultured cells.Plant molecular biology · 2000Article
- Signaling events leading to crassulacean acid metabolism induction in the common ice plantPlant physiology · 1999Article
- A Structural Bridge Between Kingdoms: How Collagen-Derived Peptides Influence Plant Stress and Growth Pathways.Physiologia plantarumArticle
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant responses to high salt stress have been studied for several decades. However, the molecular mechanisms underlying these responses still elude us. In order to understand better the molecular mechanism related to NaCl stress in plants, we initiated the cloning of a large number of NaCl-induced genes in Arabidopsis. Here, we report the cloning of a cDNA encoding a novel Ca2+-binding protein, named AtCP1, which shares sequence similarities with calmodulins. AtCP1 exhibits, in particular, a high degree of amino acid sequence homology to the Ca2+-binding loops of the EF hands of calmodulin. However, unlike calmodulin, AtCP1 appears to have only three Ca2+-binding loops. We examined Ca2+ binding of the protein by a Ca2+-dependent electrophoretic mobility shift assay. A recombinant AtCP1 protein that was expressed in Escherichia coli did show a Ca2+-dependent electrophoretic mobility shift. To gain insight into the expression of the AtCP1 gene, northern blot analysis was carried out. The AtCP1 gene had a tissue-specific expression pattern: high levels of expression in flower and root tissues and nearly undetectable levels in leaves and siliques. Also, the expression of the AtCP1 gene was induced by NaCl treatment but not by ABA treatment. Finally, subcellular localization experiments using an AtCP1:smGFP fusion gene in soybean suspension culture cells and tobacco leaf protoplasts indicate that AtCP1 is most likely a cytosolic protein.
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