ArticleThe Biochemical journal1998
Ceramide transport from endoplasmic reticulum to Golgi apparatus is not vesicle-mediated.
Article in The Biochemical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 45 citations in OpenAlex.
- Role of Intracellular Lipid Logistics in the Preferential Usage of Very Long Chain-Ceramides in Glucosylceramide.International journal of molecular sciences · 2016Article
- Ceramide transfer protein and cancer.Anti-cancer agents in medicinal chemistry · 2011Review
- The ceramide-enriched trans-Golgi compartments reorganize together with other parts of the Golgi apparatus in response to ATP-depletion.Histochemistry and cell biology · 2011Article
- Article
- Acute perturbations in Golgi organization impact de novo sphingomyelin synthesis.Traffic (Copenhagen, Denmark) · 2008Article
- Ceramide trafficking: learning from the rebels.Journal of biosciences · 2004Review
- Glycosphingolipids and cell death.Glycoconjugate journal · 2004Review
- The mitochondria-associated endoplasmic-reticulum subcompartment (MAM fraction) of rat liver contains highly active sphingolipid-specific glycosyltransferases.The Biochemical journal · 2003Article
- Sphingosine-1-phosphate phosphohydrolase in regulation of sphingolipid metabolism and apoptosis.The Journal of cell biology · 2002Article
- Mammalian selenoprotein in which selenocysteine (Sec) incorporation is supported by a new form of Sec insertion sequence element.Molecular and cellular biology · 2002Article
- Vesicular and nonvesicular transport of ceramide from ER to the Golgi apparatus in yeast.The Journal of cell biology · 2001Article
- Inositol phosphorylceramide synthase is located in the Golgi apparatus of Saccharomyces cerevisiae.Molecular biology of the cell · 2000Article
- Genetic evidence for ATP-dependent endoplasmic reticulum-to-Golgi apparatus trafficking of ceramide for sphingomyelin synthesis in Chinese hamster ovary cells.The Journal of cell biology · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ceramide (Cer) transfer from the endoplasmic reticulum (ER) to the Golgi apparatus was measured under conditions that block vesicle-mediated protein transfer. This was done either in intact cells by reducing the incubation temperature to 15 degreesC, or in streptolysin O-permeabilized cells by manipulating the intracellular environment. In both cases, Cer transfer was not inhibited, as demonstrated by the biosynthesis of ceramide monohexosides and sphingomyelin (SM) de novo from metabolically (with [14C]serine) labelled Cer. This assay is based on the knowledge that Cer is synthesized, starting from serine and palmitoyl-CoA, at the ER, whereas glycosphingolipids and SM are synthesized in the (early) Golgi apparatus. Formation of [14C]glycosphingolipids and [14C]SM was observed under conditions that block vesicle-mediated vesicular stomatitis virus glycoprotein transport. These results indicate that [14C]Cer is transferred from ER to Golgi by a non-vesicular mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.