ArticleThe Biochemical journal1998
Identification of upstream stimulatory factor as transcriptional activator of the liver promoter of the glucokinase gene.
Article in The Biochemical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 67 citations in OpenAlex.
- Multiple E-boxes in the distal promoter of the rat pyruvate carboxylase gene function as a glucose-responsive element.PloS one · 2014Article
- Characterization of the gene expression profile of heterozygous liver-specific glucokinase knockout mice at a young age.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2012Article
- The genetics of familial combined hyperlipidaemia.Nature reviews. Endocrinology · 2012Review
- Expression of the human glucokinase gene: important roles of the 5' flanking and intron 1 sequences.PloS one · 2012Article
- Mutated beta-catenin evades a microRNA-dependent regulatory loop.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Transcriptional regulation of glucose sensors in pancreatic β-cells and liver: an update.Sensors (Basel, Switzerland) · 2010Review
- FoxO1 and HNF-4 are involved in regulation of hepatic glucokinase gene expression by resveratrol.The Journal of biological chemistry · 2009Article
- Hepatocyte growth factor family negatively regulates hepatic gluconeogenesis via induction of orphan nuclear receptor small heterodimer partner in primary hepatocytes.The Journal of biological chemistry · 2009Article
- Molecular physiology of mammalian glucokinase.Cellular and molecular life sciences : CMLS · 2009Review
- Glucose increases hepatic lipase expression in HepG2 liver cells through upregulation of upstream stimulatory factors 1 and 2.Diabetologia · 2008Article
- Body mass index is associated with USF1 haplotype in Korean premenopausal women.Journal of Korean medical science · 2008Article
- Transcriptional regulation of the distal promoter of the rat pyruvate carboxylase gene by hepatocyte nuclear factor 3beta/Foxa2 and upstream stimulatory factors in insulinoma cells.The Biochemical journal · 2007Article
- Upstream transcription factor 1 gene polymorphisms are associated with high antilipolytic insulin sensitivity and show gene-gene interactions.Journal of molecular medicine (Berlin, Germany) · 2007Article
- Upstream stimulatory factor 1 associated with familial combined hyperlipidemia, LDL cholesterol, and triglycerides.Human genetics · 2005Article
- Synergistic role of specificity proteins and upstream stimulatory factor 1 in transactivation of the mouse carboxylesterase 2/microsomal acylcarnitine hydrolase gene promoter.The Biochemical journal · 2004Article
- Overexpression of c-myc in diabetic mice restores altered expression of the transcription factor genes that regulate liver metabolism.The Biochemical journal · 2002Article
- Activation of glucokinase gene expression by hepatic nuclear factor 4alpha in primary hepatocytes.The Biochemical journal · 2002Article
- Activation of protein kinase B/cAkt in hepatocytes is sufficient for the induction of expression of the gene encoding glucokinase.The Biochemical journal · 2000Article
- Characterization of the human liver fructose-1,6-bisphosphatase gene promoter.The Biochemical journal · 2000Article
- Sterol regulatory element binding protein-1c is a major mediator of insulin action on the hepatic expression of glucokinase and lipogenesis-related genes.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
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1 author at 1 institution in 1 country.
Funding
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Abstract
A functionally important cis-acting element termed P2 was identified in the liver promoter of the glucokinase gene. Element P2 was delineated by footprinting in vitro with nuclear proteins from rat liver and spleen. Its core sequence in the rat gene is a canonical CACGTG E-box. In the electrophoretic mobility-shift assay with nuclear proteins from rat liver, hepatocytes and hepatoma cells, an oligonucleotide with P2 in the context of the glucokinase promoter sequence gave rise to a DNA-protein complex shown to contain the upstream stimulatory factor (USF) by specific competition experiments and by reactivity with anti-USF antibodies. Transient transfection of hepatoma HepG2 cells, combined with site-directed mutagenesis, demonstrated that the P2 element was important for liver glucokinase promoter activity. Co-transfection of an expression plasmid coding for USF1 activated reporter gene expression in a manner dependent on an intact P2 element, whereas an expression plasmid for c-Myc was ineffective. Expression of a truncated form of USF1 lacking the transcription activation domain and the basic region decreased reporter activity by a dominant-negative effect. The functional significance of the P2 element was also demonstrated in transient transfection of primary hepatocytes.
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