ArticleBiophysical journal1998
Oligomeric state of human erythrocyte band 3 measured by fluorescence resonance energy homotransfer.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 68 citations in OpenAlex.
- Temporal sequence of major biochemical events during blood bank storage of packed red blood cells.Blood transfusion = Trasfusione del sangue · 2012Trial
- Conformational Dynamic Studies of Prokaryotic Potassium Channels Explored by Homo-FRET Methodologies.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Cow blood - A superior storage option in forensics?Heliyon · 2023Article
- Imaging of fluorescence anisotropy during photoswitching provides a simple readout for protein self-association.Nature communications · 2020Article
- Ab Initio Derivation of the FRET Equations Resolves Old Puzzles and Suggests Measurement Strategies.Biophysical journal · 2019Article
- Purinergic control of red blood cell metabolism: novel strategies to improve red cell storage quality.Blood transfusion = Trasfusione del sangue · 2017Review
- Simultaneous imaging of multiple cellular events using high-accuracy fluorescence polarization microscopy.Microscopy (Oxford, England) · 2017Article
- L30A Mutation of Phospholemman Mimics Effects of Cardiac Glycosides in Isolated Cardiomyocytes.Biochemistry · 2016Article
- Dopamine Receptor Signaling in MIN6 β-Cells Revealed by Fluorescence Fluctuation Spectroscopy.Biophysical journal · 2016Article
- Automated structure refinement for a protein heterodimer complex using limited EPR spectroscopic data and a rigid-body docking algorithm: a three-dimensional model for an ankyrin-CDB3 complex.The journal of physical chemistry. B · 2014Article
- When one plus one does not equal two: fluorescence anisotropy in aggregates and multiply labeled proteins.Biophysical journal · 2014Article
- In vivo imaging of the actin polymerization state with two-photon fluorescence anisotropy.Biophysical journal · 2012Article
- Probing orientational behavior of MHC class I protein and lipid probes in cell membranes by fluorescence polarization-resolved imaging.Biophysical journal · 2011Article
- Determination of structural models of the complex between the cytoplasmic domain of erythrocyte band 3 and ankyrin-R repeats 13-24.The Journal of biological chemistry · 2011Article
- Distance mapping in proteins using fluorescence spectroscopy: the tryptophan-induced quenching (TrIQ) method.Biochemistry · 2010Article
- Fluorescence anisotropy of protein complexes in living cells.Biophysical journal · 2010Article
- GABA transporter function, oligomerization state, and anchoring: correlates with subcellularly resolved FRET.The Journal of general physiology · 2009Article
- Homo-FRET imaging enables quantification of protein cluster sizes with subcellular resolution.Biophysical journal · 2009Article
- Changes in Band 3 oligomeric state precede cell membrane phospholipid loss during blood bank storage of red blood cells.Transfusion · 2009Article
- Enumeration of oligomerization states of membrane proteins in living cells by homo-FRET spectroscopy and microscopy: theory and application.Biophysical journal · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The oligomeric state of the erythrocyte anion exchange protein, band 3, has been assayed by resonance energy homotransfer. Homotransfer between oligomeric subunits, labeled with eosin-5-maleimide at Lys430 in the transmembrane domain, has been demonstrated by steady-state and time-resolved fluorescence spectroscopy, and is readily observed by its depolarization of the eosin fluorescence. Polarized fluorescence measurements of HPLC-purified band 3 oligomers indicate that eosin homotransfer increases progressively with increasing species size. This shows that homotransfer also occurs between labeled band 3 dimers as well as within the dimers, making fluorescence anisotropy measurements sensitive to band 3 self-association. Treatment of ghost membranes with either Zn2+ or melittin, agents that cluster band 3, significantly decreases the anisotropy as a result of the increased homotransfer within the band 3 clusters. By comparison with the anisotropy of species of known oligomeric state, the anisotropy of erythrocyte ghost membranes at 37 degrees C is consistent with dimeric and/or tetrameric band 3, and does not require postulation of a fraction of large clusters. Proteolytic removal of the cytoplasmic domain of band 3, which significantly increases the rotational mobility of the transmembrane domain, does not affect its oligomeric state, as reported by eosin homotransfer. These results support a model in which interaction with the membrane skeleton restricts the mobility of band 3 without significantly altering its self-association state.
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