ArticleBiophysical journal1998
Circular dichroism of DNA frayed wires.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Structure Validation of G-Rich RNAs in Noncoding Regions of the Human Genome.Chembiochem : a European journal of chemical biology · 2020Article
- Abolishing HIV-1 infectivity using a polypurine tract-specific G-quadruplex-forming oligonucleotide.BMC infectious diseases · 2016Article
- Circular dichroism and conformational polymorphism of DNA.Nucleic acids research · 2009Review
- Bimolecular quadruplexes and their transitions to higher-order molecular structures detected by ESI-FTICR-MS.Journal of the American Society for Mass Spectrometry · 2007Article
- Structural transition from antiparallel to parallel G-quadruplex of d(G4T4G4) induced by Ca2+.Nucleic acids research · 2003Article
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Authors and funding
2 authors.
Funding
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Abstract
Ultraviolet circular dichroism spectra are reported for the oligonucleotide d(A15G15) in aqueous solutions containing 5 mM MgCl2 at several temperatures and in the presence of partially complementary oligonucleotides. Oligonucleotides with several consecutive terminal guanine residues self-associate to form aggregates, called frayed wires, that consist of integer numbers of strands. A "stem" is formed through interactions between the guanine residues of the associated oligonucleotides, whereas the adenine "arms" remain single stranded. Upon subtracting the circular dichroism spectrum of d(A15) from that of d(A15G15), one obtains a spectrum that closely resembles previously published spectra of poly(G). Subtracting spectra measured at temperatures between 10 degrees C and 60 degrees C reveals the resultant spectra to be independent of temperature, consistent with the extreme thermal stability observed for the aggregated structures. Upon the addition of d(T15) to the solution, complexes with the adenine portion of the d(A15G15) frayed wires are formed. Subtraction of d(A15):d(T15) spectra measured at several temperatures from those of the d(A15G15):d(T15) does not significantly alter the spectrum of the guanines. The helix-coil transition temperature of d(A15):d(T15) duplex is identical to that of the unbinding of d(T15) from d(A15G15):d(T15) complexes. Experiments using oligonucleotides in which the adenines were replaced with sequences of bases yielded similar results. By varying the length of the nonguanine tract, it is shown that the solubility of the complexes increases with the length of the nonguanine region of the oligonucleotide.
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