ArticleBiophysical journal1998
Imaging of the surface of living cells by low-force contact-mode atomic force microscopy.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Chemical fixation creates nanoscale clusters on the cell surface by aggregating membrane proteins.Communications biology · 2022Article
- Quantifying molecular- to cellular-level forces in living cells.Journal of physics D: Applied physics · 2021Article
- Scanning ion conductance microscopy of isolated metaphase chromosomes in a liquid environment.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Article
- Switching behaviour in vascular smooth muscle cell-matrix adhesion during oscillatory loading.Journal of theoretical biology · 2020Article
- Application of atomic force microscope in diagnosis of single cancer cells.Biomicrofluidics · 2020Review
- Article
- Partially oxidized DJ-1 inhibits α-synuclein nucleation and remodels mature α-synuclein fibrils in vitro.Communications biology · 2019Article
- The effect of the serum corona on interactions between a single nano-object and a living cell.Scientific reports · 2017Article
- PeakForce Tapping resolves individual microvilli on living cells.Journal of molecular recognition : JMR · 2016Article
- Antiproliferative effects of cinobufacini on human hepatocellular carcinoma HepG2 cells detected by atomic force microscopy.World journal of gastroenterology · 2015Article
- Gold nanoparticles inhibit VEGF165-induced migration and tube formation of endothelial cells via the Akt pathway.BioMed research international · 2014Article
- Article
- Dynamics of leading lamellae of living fibroblasts visualized by high-speed scanning probe microscopy.Histochemistry and cell biology · 2010Article
- Force probing surfaces of living cells to molecular resolution.Nature chemical biology · 2009Review
- Changes induced by hyperosmotic mannitol in cerebral endothelial cells: an atomic force microscopic study.European biophysics journal : EBJ · 2007Article
- Cellular transfer and AFM imaging of cancer cells using Bioimprint.Journal of nanobiotechnology · 2006Article
- Control Issues in High-speed AFM for Biological Applications: Collagen Imaging Example.Asian journal of control · 2004Article
- A three-dimensional viscoelastic model for cell deformation with experimental verification.Biophysical journal · 2003Article
- Nanotechnology for neuronal ion channels.Journal of neurology, neurosurgery, and psychiatry · 2003Review
- Adhesively-tensed cell membranes: lysis kinetics and atomic force microscopy probing.Biophysical journal · 2003Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The membrane surface of living CV-1 kidney cells in culture was imaged by contact-mode atomic force microscopy using scanning forces in the piconewton range. A simple procedure was developed for imaging of the cell surface with forces as low as 20-50 pN, i.e., two orders of magnitude below those commonly used for cell imaging. Under these conditions, the indentation of the cells by the tip could be reduced to less than l0 nm, even at the cell center, which gave access to the topographic image of the cell surface. This surface appeared heterogeneous with very few villosities and revealed, only in distinct areas, the submembrane cytoskeleton. At intermediate magnifications, corresponding to 20-5 microm scan sizes, the surface topography likely reflected the organization of submembrane and intracellular structures on which the plasma membrane lay. By decreasing the scan size, a lateral resolution better than 20 nm was routinely obtained for the cell surface, and a lateral resolution better than 10 nm was obtained occasionally. The cell surface appeared granular, with packed particles, likely corresponding to proteins or protein-lipid complexes, between approximately 5 and 30 nm xy size.
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