ArticleBiophysical journal1998
Extending the range of rate constants available from BIACORE: interpreting mass transport-influenced binding data.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
78 citing papers in PubMed.
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- A Design Strategy for Surface Nanostructures to Realize Sensitive Refractive-Index Optical Sensors.Nanomaterials (Basel, Switzerland) · 2023Article
- Effect of Gravity on Bacterial Adhesion to Heterogeneous Surfaces.Pathogens (Basel, Switzerland) · 2023Article
- Protein Interaction Analysis by Surface Plasmon Resonance.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Applications of Surface Plasmon Resonance and Biolayer Interferometry for Virus-Ligand Binding.Viruses · 2022Review
- Smartphone-based Surface Plasmon Resonance Sensors: a Review.Plasmonics (Norwell, Mass.) · 2022Review
- Stochastic Time Response and Ultimate Noise Performance of Adsorption-Based Microfluidic Biosensors.Biosensors · 2021Article
- A rebinding-assay for measuring extreme kinetics using label-free biosensors.Scientific reports · 2021Article
- High-Sensitivity High-Throughput Detection of Nucleic Acid Targets on Metasurface Fluorescence Biosensors.Biosensors · 2021Article
- Temporal response of biochemical and biological sensors with bimodal surface adsorption from a finite sample.Microsystem technologies : sensors, actuators, systems integration · 2021Article
- Unlocking latent kinetic information from label-free binding.Scientific reports · 2019Article
- A walk through tau therapeutic strategies.Acta neuropathologica communications · 2019Review
- Characterization of receptor binding kinetics for vascular endothelial growth factor-A using SPR.Analytical biochemistry · 2019Article
- Receptor heterogeneity in optical biosensors.Journal of mathematical biology · 2018Article
- Article
18 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
Abstract
Surface-based binding assays are often influenced by the transport of analyte to the sensor surface. Using simulated data sets, we test a simple two-compartment model to see if its description of transport and binding is sufficient to accurately analyze BIACORE data. First we present a computer model that can generate realistic BIACORE data. This model calculates the laminar flow of analyte within the flow cell, its diffusion both perpendicular and parallel to the sensor surface, and the reversible chemical reaction between analyte and immobilized reactant. We use this computer model to generate binding data under a variety of conditions. An analysis of these data sets with the two-compartment model demonstrates that good estimates of the intrinsic reaction rate constants are recovered even when mass transport influences the binding reaction. We also discuss the conditions under which the two-compartment model can be used to determine the diffusion coefficient of the analyte. Our results illustrate that this model can significantly extend the range of association rate constants that can be accurately determined from BIACORE.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.