ArticleInfection and immunity1998
Vector development for the expression of foreign proteins in the vaccine strain Brucella abortus S19.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Article
- Brucella abortus S19 GFP-tagged vaccine allows the serological identification of vaccinated cattle.PloS one · 2021Article
- GFP tagging of Brucella melitensis Rev1 allows the identification of vaccinated sheep.Transboundary and emerging diseases · 2019Article
- Article
- In search of Brucella abortus type IV secretion substrates: screening and identification of four proteins translocated into host cells through VirB system.Cellular microbiology · 2011Article
- Brucellosis: the case for live, attenuated vaccines.Vaccine · 2009Review
- A B lymphocyte mitogen is a Brucella abortus virulence factor required for persistent infection.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- The Brucella abortus cyclic beta-1,2-glucan virulence factor is substituted with O-ester-linked succinyl residues.Journal of bacteriology · 2006Article
- Brucella abortus synthesizes phosphatidylcholine from choline provided by the host.Journal of bacteriology · 2006Article
- Molecular cloning and characterization of cgt, the Brucella abortus cyclic beta-1,2-glucan transporter gene, and its role in virulence.Infection and immunity · 2004Article
- Brucella abortus cyclic beta-1,2-glucan mutants have reduced virulence in mice and are defective in intracellular replication in HeLa cells.Infection and immunity · 2001Article
- A homologue of an operon required for DNA transfer in Agrobacterium is required in Brucella abortus for virulence and intracellular multiplication.Journal of bacteriology · 2000Article
- Brucella abortus strain RB51 as a vector for heterologous protein expression and induction of specific Th1 type immune responses.Infection and immunity · 2000Article
- Molecular cloning and characterization of cgs, the Brucella abortus cyclic beta(1-2) glucan synthetase gene: genetic complementation of Rhizobium meliloti ndvB and Agrobacterium tumefaciens chvB mutants.Journal of bacteriology · 1998Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
A vector for the expression of foreign antigens in the vaccine strain Brucella abortus S19 was developed by using a DNA fragment containing the regulatory sequences and the signal peptide of the Brucella bcsp31 gene. This fragment was cloned in broad-host-range plasmid pBBR4MCS, resulting in plasmid pBEV. As a reporter protein, a repetitive antigen of Trypanosoma cruzi was used. The recombinant fusion protein is stably expressed and secreted into the Brucella periplasmic space, inducing a good antibody response against the T. cruzi antigen. The expression of the repetitive antigen in Brucella neither altered its growth pattern nor generated a toxic or lethal effect during experimental infection. The application of this strategy for the generation of live recombinant vaccines and the tagging of B. abortus S19 vaccine is discussed. This is the first time that a recombinant protein has been expressed in the periplasm of brucellae.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.