ArticleNucleic acids research1998
The -16 region of Bacillus subtilis and other gram-positive bacterial promoters.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 113 citations in OpenAlex.
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- Promoter DNA recognition by theFrontiers in molecular biosciences · 2023Article
- The bacterial promoter spacer modulates promoter strength and timing by length, TG-motifs and DNA supercoiling sensitivity.Scientific reports · 2021Article
- Application of Bacillus subtilis as a live vaccine vector: A review.The Journal of veterinary medical science · 2020Review
- Integrative characterization of the near-minimal bacterium Mesoplasma florum.Molecular systems biology · 2020Article
- Natural and engineered promoters for gene expression in Lactobacillus species.Applied microbiology and biotechnology · 2020Review
- Optogenetic control of Bacillus subtilis gene expression.Nature communications · 2019Article
- RbpA relaxes promoter selectivity of M. tuberculosis RNA polymerase.Nucleic acids research · 2018Article
- σJournal of bacteriology · 2018Article
- Tinkering with Osmotically Controlled Transcription Allows Enhanced Production and Excretion of Ectoine and Hydroxyectoine from a Microbial Cell Factory.Applied and environmental microbiology · 2018Article
- Development of an efficient autoinducible expression system by promoter engineering in Bacillus subtilis.Microbial cell factories · 2016Article
- Rapid Detection of Viable Bacillus anthracis Spores in Environmental Samples by Using Engineered Reporter Phages.Applied and environmental microbiology · 2016Article
- Bacterial antisense RNAs are mainly the product of transcriptional noise.Science advances · 2016Article
- Distinguishing between productive and abortive promoters using a random forest classifier in Mycoplasma pneumoniae.Nucleic acids research · 2015Article
- Transcription of the Streptococcus pyogenes hyaluronic acid capsule biosynthesis operon is regulated by previously unknown upstream elements.Infection and immunity · 2014Article
- Article
- Mutational analysis of cj0183 Campylobacter jejuni promoter.Current microbiology · 2013Article
- Identification and expression of genes involved in the conversion of daidzein and genistein by the equol-forming bacterium Slackia isoflavoniconvertens.Applied and environmental microbiology · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Bacillus subtilis alpha-amylase promoter amy P contains an essential TGTG motif (-16 region) upstream of the -10 region. Mutations of this region significantly reduced in vitro promoter strength. A -15 G-->C transversion eliminated transcription from amy P by both B.subtilis and Escherichia coli RNA polymerase (RNAP). A second alpha-amylase promoter ( amy P2) also required the -16 region for function. To determine conserved sequences in promoters containing -16 region elements, sequences of 64 B.subtilis promoters with the second TG motif of the -16 region were aligned and analyzed. Unlike the E.coli class of 'extended -10 promoters', with a similar TG motif but lacking a -35 region, the -16 region promoters contain highly conserved -35 regions. They also contain conserved A n and T n tracts upstream of the -35 region. In addition, we analyzed all available gram-positive bacterial promoter compilations to determine the generality of the -16 region. From this analysis, the -16 region TRTG motif (R = purine) appears to be a basic element found in a large portion of gram-positive bacterial promoters and is, in the case of at least the alpha-amylase promoters, necessary for transcription by the major form of B.subtilis and E.coli RNAP.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.