ArticleNucleic acids research1998
Chemical display of thymine residues flipped out by DNA methyltransferases.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 42 citations in OpenAlex.
- High-precision mapping reveals rare NCell discovery · 2022Article
- DNA base flipping analytical pipeline.Biology methods & protocols · 2017Article
- Chemical display of pyrimidine bases flipped out by modification-dependent restriction endonucleases of MspJI and PvuRts1I families.PloS one · 2014Article
- Direct observation of cytosine flipping and covalent catalysis in a DNA methyltransferase.Nucleic acids research · 2011Article
- Article
- Ground state intermolecular proton transfer in the supersystems thymine-(H2O)n and thymine-(CH3OH)n, n = 1,2: a theoretical study.Journal of molecular modeling · 2009Article
- Chemical mapping of cytosines enzymatically flipped out of the DNA helix.Nucleic acids research · 2008Article
- Base-flipping dynamics in a DNA hairpin processing reaction.Nucleic acids research · 2007Article
- Molecular architecture of a eukaryotic DNA replication terminus-terminator protein complex.Molecular and cellular biology · 2006Article
- KpnI restriction endonuclease and methyltransferase exhibit contrasting mode of sequence recognition.Nucleic acids research · 2004Article
- Sequence-structure-function studies of tRNA:m5C methyltransferase Trm4p and its relationship to DNA:m5C and RNA:m5U methyltransferases.Nucleic acids research · 2004Article
- AdoMet-dependent methylation, DNA methyltransferases and base flipping.Nucleic acids research · 2001Review
- A dual role for substrate S-adenosyl-L-methionine in the methylation reaction with bacteriophage T4 Dam DNA-[N6-adenine]-methyltransferase.Nucleic acids research · 2001Article
- Substrate binding in vitro and kinetics of RsrI [N6-adenine] DNA methyltransferase.Nucleic acids research · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The DNA cytosine-C5 methyltransferase M. Hha I flips its target base out of the DNA helix during interaction with the substrate sequence GCGC. Binary and ternary complexes between M. Hha I and hemimethylated DNA duplexes were used to examine the suitability of four chemical methods to detect flipped-out bases in protein-DNA complexes. These methods probe the structural peculiarities of pyrimidine bases in DNA. We find that in cases when the target cytosine is replaced with thymine (GTGC), KMnO4proved an efficient probe for positive display of flipped-out thymines. The generality of this procedure was further verified by examining a DNA adenine-N6 methyltransferase, M. Taq I, in which case an enhanced reactivity of thymine replacing the target adenine (TCGT) in the recognition sequence TCGA was also observed. Our results support the proposed base-flipping mechanism for adenine methyltransferases, and offer a convenient laboratory tool for detection of flipped-out thymines in protein-DNA complexes.
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