ArticleProceedings of the National Academy of Sciences of the United States of America1998
Evidence for gene silencing by endogenous DNA methylation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Who cites it
12 citing papers in PubMed.
- Using Selective Enzymes to Measure Noncanonical DNA Building Blocks: dUTP, 5-Methyl-dCTP, and 5-Hydroxymethyl-dCTP.Biomolecules · 2023Article
- Epigenetic Pyrimidine Nucleotides in Competition with Natural dNTPs as Substrates for Diverse DNA Polymerases.ACS chemical biology · 2022Article
- Analysis of the gene transcription patterns and DNA methylation characteristics of triploid sea cucumbers (Apostichopus japonicus).Scientific reports · 2021Article
- Article
- Evolutionary Basis of Human Running and Its Impact on Neural Function.Frontiers in systems neuroscience · 2016Review
- DREAMing of a patent-free human genome for clinical sequencing.Nature biotechnology · 2013Article
- Social allostasis: anticipatory regulation of the internal milieu.Frontiers in evolutionary neuroscience · 2011Article
- Article
- Crypt dynamics and colorectal cancer: advances in mathematical modelling.Cell proliferation · 2006Article
- Epigenetics comes of age in the twentyfirst century.Journal of genetics · 2002Article
- A sense of self: the role of DNA sequence elimination in allopolyploidization.The Plant cell · 2001Article
- Genome evolution in polyploids.Plant molecular biology · 2000Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transformed cells can spontaneously silence genes by de novo methylation, and it is generally assumed that this is due to DNA methyltransferase activity. We have tested the alternative hypothesis that gene silencing could be due to the uptake of 5-methyl-dCMP into DNA, via the di- and triphosphonucleotides. 5-Methyl-dCMP would be present in cells from the ongoing repair of DNA. We have isolated a strain of Chinese hamster ovary (CHO) cells, designated HAM-, which spontaneously silences two tested genes at a very high frequency. We have shown that this strain incorporates 5-[3H]methyldeoxycytidine into 5-methylcytosine and thymine in DNA. It also has low 5-methyl-dCMP deaminase activity. Another HAM+ strain has high deaminase activity and a very low frequency of gene silencing. The starting strain, CHO K1, has a phenotype intermediate between HAM- and HAM+.
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