ArticleMolecular and cellular biology1998
Nuclear proteins Nut1p and Nut2p cooperate to negatively regulate a Swi4p-dependent lacZ reporter gene in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 53 citations in OpenAlex.
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- Role of Mediator in regulating Pol II elongation and nucleosome displacement in Saccharomyces cerevisiae.Genetics · 2012Article
- Origins and activity of the Mediator complex.Seminars in cell & developmental biology · 2011Review
- The transcriptional coactivator DRIP/mediator complex is involved in vitamin D receptor function and regulates keratinocyte proliferation and differentiation.The Journal of investigative dermatology · 2010Article
- Differential roles of transcriptional mediator subunits in regulation of multidrug resistance gene expression in Saccharomyces cerevisiae.Molecular biology of the cell · 2010Article
- Dissection of coactivator requirement at RNR3 reveals unexpected contributions from TFIID and SAGA.The Journal of biological chemistry · 2008Article
- A functional module of yeast mediator that governs the dynamic range of heat-shock gene expression.Genetics · 2006Article
- Genetic interactions between mediator and the late G1-specific transcription factor Swi6 in Saccharomyces cerevisiae.Genetics · 2005Article
- Characterization of yeast histone H3-specific type B histone acetyltransferases identifies an ADA2-independent Gcn5p activity.BMC biochemistry · 2004Article
- A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Use of an in vivo reporter assay to test for transcriptional and translational fidelity in yeast.The Journal of biological chemistry · 2002Article
- Histone H3 specific acetyltransferases are essential for cell cycle progression.Genes & development · 2001Article
- Multiple regulators of Ty1 transposition in Saccharomyces cerevisiae have conserved roles in genome maintenance.Genetics · 2001Article
- Functional interaction of CCR4-NOT proteins with TATAA-binding protein (TBP) and its associated factors in yeast.Genetics · 2000Article
- Activator-specific requirement of yeast mediator proteins for RNA polymerase II transcriptional activation.Molecular and cellular biology · 1999Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The URS2 region of the Saccharomyces cerevisiae HO upstream region contains 10 binding sites for the Swi4p/Swi6p transcription factor and confers Swi4p dependence for transcription. Using a hybrid promoter, UASGAL (upstream activation sequence of GAL1)-URS2R, in which the GAL1-10 regulatory region is fused to the proximal 360 bp of URS2, we isolated mutants in which Swi4p is no longer required for transcription. Mutations of SIN4, ROX3, SRB8, SRB9, SRB10, SRB11, and two novel genes, NUT1 and NUT2, relieve the requirement of Swi4p for expression of this reporter. We found that NUT1 (open reading frame [ORF] YGL151w) is a nonessential gene, that NUT2 (ORF YPR168w) is essential, and that both Nut1p and Nut2p encode nuclear proteins. Deletion of NUT1 causes a constitutive, Swi4p-independent phenotype only in combination with the nut2-1 allele or an allele of CCR4. In contrast, inactivation of a temperature-sensitive allele of NUT2, nut2-ts70, alone causes constitutivity. nut1Delta nut2-1 cells and sin4Delta cells exhibit Swi4p-independent expression of an ho-lacZ reporter but not of an intact ho gene. Likewise, a pPHO5-lacZ construct is constitutively expressed in nut1 nut2 mutants relative to their wild-type counterparts. These results suggest that Nut1p, Nut2p, Sin4p, and Ccr4p define a group of proteins that negatively regulate transcription in a subtle manner which is revealed by artificial reporter genes.
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