ArticleMolecular and cellular biology1998
Exonic sequences in the 5' untranslated region of alpha-tubulin mRNA modulate trans splicing in Trypanosoma brucei.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- TheRNA (New York, N.Y.) · 2022Article
- Regulation of gene expression in trypanosomatids: living with polycistronic transcription.Open biology · 2019Article
- Article
- Genetic structure and expression of the surface glycoprotein GP82, the main adhesin of Trypanosoma cruzi metacyclic trypomastigotes.TheScientificWorldJournal · 2013Review
- Gene expression in Trypanosoma brucei: lessons from high-throughput RNA sequencing.Trends in parasitology · 2011Review
- Cell biology of the trypanosome genome.Microbiology and molecular biology reviews : MMBR · 2010Review
- The transcriptome of the human pathogen Trypanosoma brucei at single-nucleotide resolution.PLoS pathogens · 2010Article
- Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.Nucleic acids research · 2010Article
- Establishment of an in vitro trans-splicing system in Trypanosoma brucei that requires endogenous spliced leader RNA.Nucleic acids research · 2010Article
- Different trans RNA splicing events in bloodstream and procyclic Trypanosoma brucei.Molecular and biochemical parasitology · 2008Article
- Evolution of tubulin gene arrays in Trypanosomatid parasites: genomic restructuring in Leishmania.BMC genomics · 2006Article
- Comparative genomics and concerted evolution of beta-tubulin paralogs in Leishmania spp.BMC genomics · 2006Article
- Systematic study of sequence motifs for RNA trans splicing in Trypanosoma brucei.Molecular and cellular biology · 2005Article
- A computational investigation of kinetoplastid trans-splicing.Genome biology · 2005Article
- Spliced-leader RNA trans splicing in a chordate, Oikopleura dioica, with a compact genome.Molecular and cellular biology · 2004Article
- An organism-specific method to rank predicted coding regions in Trypanosoma brucei.Nucleic acids research · 2003Article
- trans and cis splicing in trypanosomatids: mechanism, factors, and regulation.Eukaryotic cell · 2003Review
- A complex containing CstF-64 and the SL2 snRNP connects mRNA 3' end formation and trans-splicing in C. elegans operons.Genes & development · 2001Article
- Functional selection of splicing enhancers that stimulate trans-splicing in vitro.RNA (New York, N.Y.) · 2001Article
- 3' splice site recognition in nematode trans-splicing involves enhancer-dependent recruitment of U2 snRNP.RNA (New York, N.Y.) · 2001Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Previous studies have identified a conserved AG dinucleotide at the 3' splice site (3'SS) and a polypyrimidine (pPy) tract that are required for trans splicing of polycistronic pre-mRNAs in trypanosomatids. Furthermore, the pPy tract of the Trypanosoma brucei alpha-tubulin 3'SS region is required to specify accurate 3'-end formation of the upstream beta-tubulin gene and trans splicing of the downstream alpha-tubulin gene. Here, we employed an in vivo cis competition assay to determine whether sequences other than those of the AG dinucleotide and the pPy tract were required for 3'SS identification. Our results indicate that a minimal alpha-tubulin 3'SS, from the putative branch site region to the AG dinucleotide, is not sufficient for recognition by the trans-splicing machinery and that polyadenylation is strictly dependent on downstream trans splicing. We show that efficient use of the alpha-tubulin 3'SS is dependent upon the presence of exon sequences. Furthermore, beta-tubulin, but not actin exon sequences or unrelated plasmid sequences, can replace alpha-tubulin exon sequences for accurate trans-splice-site selection. Taken together, these results support a model in which the informational content required for efficient trans splicing of the alpha-tubulin pre-mRNA includes exon sequences which are involved in modulation of trans-splicing efficiency. Sequences that positively regulate trans splicing might be similar to cis-splicing enhancers described in other systems.
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