ArticleMolecular and cellular biology1998
Muscle-specific splicing of a heterologous exon mediated by a single muscle-specific splicing enhancer from the cardiac troponin T gene.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
- Fine-tuning striated muscle performance: conserved sarcomere-level mechanisms across insect and vertebrate systems.Frontiers in physiology · 2026Review
- Neuron-specific repression of alternative splicing by the conserved CELF protein UNC-75 in Caenorhabditis elegans.Genetics · 2025Article
- Reversal of RNA toxicity in myotonic dystrophy via a decoy RNA-binding protein with high affinity for expanded CUG repeats.Nature biomedical engineering · 2022Article
- Alternative Pre-mRNA Splicing of the Mu Opioid Receptor Gene,Biomolecules · 2021Review
- Developmental Transcriptomics of the Hawaiian Anchialine Shrimp Halocaridina rubra Holthuis, 1963 (Crustacea: Atyidae).Integrative and comparative biology · 2016Article
- Multiple functional polymorphisms in the G6PC2 gene contribute to the association with higher fasting plasma glucose levels.Diabetologia · 2013Article
- Analysis of exonic regions involved in nuclear localization, splicing activity, and dimerization of Muscleblind-like-1 isoforms.The Journal of biological chemistry · 2011Article
- The neurofibromatosis type I pre-mRNA is a novel target of CELF protein-mediated splicing regulation.Nucleic acids research · 2010Article
- Aberrant splicing of an alternative exon in the Drosophila troponin-T gene affects flight muscle development.Genetics · 2007Article
- A correlation with exon expression approach to identify cis-regulatory elements for tissue-specific alternative splicing.Nucleic acids research · 2007Article
- Identification of CELF splicing activation and repression domains in vivo.Nucleic acids research · 2005Article
- Muscleblind proteins regulate alternative splicing.The EMBO journal · 2004Article
- ETR-3 and CELF4 protein domains required for RNA binding and splicing activity in vivo.Nucleic acids research · 2004Article
- Antagonistic regulation of alpha-actinin alternative splicing by CELF proteins and polypyrimidine tract binding protein.RNA (New York, N.Y.) · 2003Article
- Finding signals that regulate alternative splicing in the post-genomic era.Genome biology · 2002Review
- A splicing silencer that regulates smooth muscle specific alternative splicing is active in multiple cell types.Nucleic acids research · 2002Article
- Degeneracy and complexity in biological systems.Proceedings of the National Academy of Sciences of the United States of America · 2001Review
- The CELF family of RNA binding proteins is implicated in cell-specific and developmentally regulated alternative splicing.Molecular and cellular biology · 2001Article
- Multiple splicing defects in an intronic false exon.Molecular and cellular biology · 2000Article
- Requirements for mini-exon inclusion in potato invertase mRNAs provides evidence for exon-scanning interactions in plants.RNA (New York, N.Y.) · 2000Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chicken cardiac troponin T (cTNT) gene contains a single 30-nucleotide alternative exon that is included in embryonic striated muscle and skipped in the adult. Transient-transfection analysis of cTNT minigenes in muscle and fibroblast cell cultures previously identified four muscle-specific splicing enhancers (MSEs) that promote exon inclusion specifically in embryonic striated muscle cultures. Three MSEs located in the intron downstream from the alternative exon were sufficient for muscle-specific exon inclusion. In the present study, the boundaries of these MSEs were defined by scanning mutagenesis, allowing analysis of individual elements in gain-of-function experiments. Concatamers of MSE2 were necessary and sufficient to promote muscle-specific inclusion of a heterologous exon, indicating that it is a target for muscle-specific regulation. Sequences present in MSE2 are also found in MSE4, suggesting that these two MSEs act in a similar manner. MSE3 appears to be different from MSE2 and MSE4 yet is able to functionally replace both of these elements, demonstrating functional redundancy of elements that are likely to bind different factors. MSE2 and MSE4 each contain a novel sequence motif that is found adjacent to a number of alternative exons that undergo regulated splicing in striated muscle, suggesting a common role for this element in muscle-specific regulation.
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