ArticleMolecular and cellular biology1998
Interplay of positive and negative regulators in transcription initiation by RNA polymerase II holoenzyme.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 103 citations in OpenAlex.
- The CCR4-NOT complex: a multifaceted sensor of molecular signals instructing eukaryotic mRNA translation and stability.Nucleic acids research · 2025Review
- Architectural Mediator subunits are differentially essential for global transcription in Saccharomyces cerevisiae.Genetics · 2021Article
- Review
- Role of integrative structural biology in understanding transcriptional initiation.Methods (San Diego, Calif.) · 2019Review
- Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex.Biochemistry research international · 2018Article
- Article
- Multifunctional roles of the mammalian CCR4-NOT complex in physiological phenomena.Frontiers in genetics · 2014Review
- Insights into the structure and architecture of the CCR4-NOT complex.Frontiers in genetics · 2014Review
- Mediator is an intrinsic component of the basal RNA polymerase II machinery in vivo.Nucleic acids research · 2013Article
- Structure of the Mediator head module.Nature · 2012Article
- Mediator head subcomplex Med11/22 contains a common helix bundle building block with a specific function in transcription initiation complex stabilization.Nucleic acids research · 2011Article
- Drosophila TIEG is a modulator of different signalling pathways involved in wing patterning and cell proliferation.PloS one · 2011Article
- Crystal structure of the human CNOT6L nuclease domain reveals strict poly(A) substrate specificity.The EMBO journal · 2010Article
- The structural basis for deadenylation by the CCR4-NOT complex.Protein & cell · 2010Review
- The yeast RNA polymerase II-associated factor Iwr1p is involved in the basal and regulated transcription of specific genes.The Journal of biological chemistry · 2009Article
- Article
- Cooperative action of NC2 and Mot1p to regulate TATA-binding protein function across the genome.Genes & development · 2008Article
- Global distribution of negative cofactor 2 subunit-alpha on human promoters.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Article
- Regulation of histone H3K4 tri-methylation and PAF complex recruitment by the Ccr4-Not complex.Nucleic acids research · 2007Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Activation of protein-encoding genes involves recruitment of an RNA polymerase II holoenzyme to promoters. Since the Srb4 subunit of the holoenzyme is essential for expression of most class II genes and is a target of at least one transcriptional activator, we reasoned that suppressors of a temperature-sensitive mutation in Srb4 would identify other factors generally involved in regulation of gene expression. We report here that MED6 and SRB6, both of which encode essential components of the holoenzyme, are among the dominant suppressors and that the products of these genes interact physically with Srb4. The recessive suppressors include NCB1 (BUR6), NCB2, NOT1, NOT3, NOT5, and CAF1, which encode subunits of NC2 and the Not complex. NC2 and Not proteins are general negative regulators which interact with TATA box binding protein (TBP). Taken together, these results suggest that transcription initiation involves a dynamic balance between activation mediated by specific components of the holoenzyme and repression by multiple TBP-associated regulators.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.