ArticleRNA (New York, N.Y.)1998
Translational activation of uncapped mRNAs by the central part of human eIF4G is 5' end-dependent.
Article in RNA (New York, N.Y.), 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
48 citing papers in PubMed, 86 citations in OpenAlex.
- Photocaged 5' cap analogues for optical control of mRNA translation in cells.Nature chemistry · 2022Article
- Ribosomal leaky scanning through a translated uORF requires eIF4G2.Nucleic acids research · 2022Article
- Biomedical applications of mRNA nanomedicine.Nano research · 2018Article
- microRNAs stimulate translation initiation mediated by HCV-like IRESes.Nucleic acids research · 2017Article
- Eukaryotic translation initiation factor 4G (eIF4G) coordinates interactions with eIF4A, eIF4B, and eIF4E in binding and translation of the barley yellow dwarf virus 3' cap-independent translation element (BTE).The Journal of biological chemistry · 2017Article
- A researcher's guide to the galaxy of IRESs.Cellular and molecular life sciences : CMLS · 2017Review
- Structure of human IFIT1 with capped RNA reveals adaptable mRNA binding and mechanisms for sensing N1 and N2 ribose 2'-O methylations.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Toward the mechanism of eIF4F-mediated ribosomal attachment to mammalian capped mRNAs.Genes & development · 2016Article
- Inhibition of Mitogen-activated Protein Kinase (MAPK)-interacting Kinase (MNK) Preferentially Affects Translation of mRNAs Containing Both a 5'-Terminal Cap and Hairpin.The Journal of biological chemistry · 2016Article
- Upstream Open Reading Frames Located in the Leader of Protein Kinase Mζ mRNA Regulate Its Translation.Frontiers in molecular neuroscience · 2016Article
- Rolling Circle Translation of Circular RNA in Living Human Cells.Scientific reports · 2015Article
- Article
- Mextli is a novel eukaryotic translation initiation factor 4E-binding protein that promotes translation in Drosophila melanogaster.Molecular and cellular biology · 2013Article
- Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A.Structure (London, England : 1993) · 2013Article
- A novel mechanism of eukaryotic translation initiation that is neither m7G-cap-, nor IRES-dependent.Nucleic acids research · 2013Article
- Poly(A)-binding protein facilitates translation of an uncapped/nonpolyadenylated viral RNA by binding to the 3' untranslated region.Journal of virology · 2012Article
- Cap-dependent translation without base-by-base scanning of an messenger ribonucleic acid.Nucleic acids research · 2012Article
- A cellular response linking eIF4AI activity to eIF4AII transcription.RNA (New York, N.Y.) · 2012Article
- Specific domains in yeast translation initiation factor eIF4G strongly bias RNA unwinding activity of the eIF4F complex toward duplexes with 5'-overhangs.The Journal of biological chemistry · 2012Article
- Angiogenin-induced tRNA fragments inhibit translation initiation.Molecular cell · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Translation initiation factor (eIF) 4G represents a critical link between mRNAs and 40S ribosomal subunits during translation initiation. It interacts directly with the cap-binding protein eIF4E through its N-terminal part, and binds eIF3 and eIF4A through the central and C-terminal region. We expressed and purified recombinant variants of human eIF4G lacking the N-terminal domain as GST-fusion proteins, and studied their function in cell-free translation reactions. Both eIF4G lacking its N-terminal part (aa 486-1404) and the central part alone (aa 486-935) exert a dominant negative effect on the translation of capped mRNAs. Furthermore, these polypeptides potently stimulate the translation of uncapped mRNAs. Although this stimulation is cap-independent, it is shown to be dependent on the accessibility of the mRNA 5' end. These results reveal two unexpected features of eIF4G-mediated translation. First, the C-terminal eIF4A binding site is dispensable for activation of uncapped mRNA translation. Second, translation of uncapped mRNA still requires 5' end-dependent ribosome binding. These new findings are incorporated into existing models of mammalian translation initiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.