ArticleThe EMBO journal1998
Chimeric purine transporters of Aspergillus nidulans define a domain critical for function and specificity conserved in bacterial, plant and metazoan homologues.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 72 citations in OpenAlex.
- Molecular Regulation and Evolution of Redox Homeostasis in Photosynthetic Machinery.Antioxidants (Basel, Switzerland) · 2022Review
- Comparative Genomics, Whole-Genome Re-sequencing and Expression Profile Analysis of Nucleobase:Cation Symporter 2 (Frontiers in plant science · 2018Article
- Understanding transporter specificity and the discrete appearance of channel-like gating domains in transporters.Frontiers in pharmacology · 2014Review
- Substrate selectivity of YgfU, a uric acid transporter from Escherichia coli.The Journal of biological chemistry · 2012Article
- Insights to the evolution of Nucleobase-Ascorbate Transporters (NAT/NCS2 family) from the Cys-scanning analysis of xanthine permease XanQ.International journal of biochemistry and molecular biology · 2012Article
- Article
- The role of transmembrane segment TM3 in the xanthine permease XanQ of Escherichia coli.The Journal of biological chemistry · 2011Article
- Cysteine-scanning analysis of helices TM8, TM9a, and TM9b and intervening loops in the YgfO xanthine permease: a carboxyl group is essential at ASP-276.The Journal of biological chemistry · 2010Article
- Purine substrate recognition by the nucleobase-ascorbate transporter signature motif in the YgfO xanthine permease: ASN-325 binds and ALA-323 senses substrate.The Journal of biological chemistry · 2010Article
- Identification and functional characterization of the first nucleobase transporter in mammals: implication in the species difference in the intestinal absorption mechanism of nucleobases and their analogs between higher primates and other mammals.The Journal of biological chemistry · 2010Article
- Role of intramembrane polar residues in the YgfO xanthine permease: HIS-31 and ASN-93 are crucial for affinity and specificity, and ASP-304 and GLU-272 are irreplaceable.The Journal of biological chemistry · 2009Article
- Purine utilization by Klebsiella oxytoca M5al: genes for ring-oxidizing and -opening enzymes.Journal of bacteriology · 2009Article
- Ras GTPase-activating protein regulation of actin cytoskeleton and hyphal polarity in Aspergillus nidulans.Eukaryotic cell · 2008Article
- Inactivation of the FCY2 gene encoding purine-cytosine permease promotes cross-resistance to flucytosine and fluconazole in Candida lusitaniae.Antimicrobial agents and chemotherapy · 2005Article
- Uric acid is a genuine metabolite of Penicillium cyclopium and stimulates the expression of alkaloid biosynthesis in this fungus.Applied and environmental microbiology · 2002Article
- New plasmid system to select for Saccharomyces cerevisiae purine-cytosine permease affinity mutants.Journal of bacteriology · 2001Article
- Functional characterization of a maize purine transporter by expression in Aspergillus nidulans.The Plant cell · 2001Article
- A new family of high-affinity transporters for adenine, cytosine, and purine derivatives in Arabidopsis.The Plant cell · 2000Article
- 16th SMYTE (Small Meeting on Yeast Transport and Energetics). Casta-Papiernicka, Slovakia, September 23-27, 1998. Abstracts.Folia microbiologica · 1999Article
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Authors and funding
5 authors at 4 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Aspergillus nidulans, purine uptake is mediated by three transporter proteins: UapA, UapC and AzgA. UapA and UapC have partially overlapping functions, are 62% identical and have nearly identical predicted topologies. Their structural similarity is associated with overlapping substrate specificities; UapA is a high-affinity, high-capacity specific xanthine/uric acid transporter. UapC is a low/moderate-capacity general purine transporter. We constructed and characterized UapA/UapC, UapC/UapA and UapA/UapC/UapA chimeric proteins and UapA point mutations. The region including residues 378-446 in UapA (336-404 in UapC) has been shown to be critical for purine recognition and transport. Within this region, we identified: (i) one amino acid residue (A404) important for transporter function but probably not for specificity and two residues (E412 and R414) important for UapA function and specificity; and (ii) a sequence, (F/Y/S)X(Q/E/P) NXGXXXXT(K/R/G), which is highly conserved in all homologues of nucleobase transporters from bacteria to man. The UapC/UapA series of chimeras behaves in a linear pattern and leads to an univocal assignment of functional domains while the analysis of the reciprocal and 'sandwich' chimeras revealed unexpected inter-domain interactions. cDNAs coding for transporters including the specificity region defined by these studies have been identified for the first time in the human and Caenorhabditis elegans databases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.