ArticleJournal of clinical microbiology1998
5' nuclease PCR assay to detect Yersinia pestis.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 92 citations in OpenAlex.
- Establishment and application of a quadruple target real-time PCR assay for detecting Yersinia pestis.PloS one · 2026Article
- Multiplex PCR System for the Diagnosis of Plague.Current molecular medicine · 2025Review
- Applications of polymerase chain reaction-based methods for the diagnosis of plague (Review).Experimental and therapeutic medicine · 2022Review
- Review
- Human plague: An old scourge that needs new answers.PLoS neglected tropical diseases · 2020Review
- Genomic Insights into a New Citrobacter koseri Strain Revealed Gene Exchanges with the Virulence-Associated Yersinia pestis pPCP1 Plasmid.Frontiers in microbiology · 2016Article
- Yersinia pestis plasminogen activator gene homolog in rat tissues.Emerging infectious diseases · 2013Article
- Evaluation of inhibitor-resistant real-time PCR methods for diagnostics in clinical and environmental samples.PloS one · 2013Article
- Evidence of Yersinia pestis DNA from fleas in an endemic plague area of Zambia.BMC research notes · 2012Article
- Rapid and sensitive detection of Yersinia pestis using amplification of plague diagnostic bacteriophages monitored by real-time PCR.PloS one · 2010Article
- Ambient stable quantitative PCR reagents for the detection of Yersinia pestis.PLoS neglected tropical diseases · 2010Article
- The Yersinia pestis caf1M1A1 fimbrial capsule operon promotes transmission by flea bite in a mouse model of bubonic plague.Infection and immunity · 2009Article
- Simultaneous real-time PCR detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2007Article
- Rapid quantitative detection ofSensors and actuators. B, Chemical · 2006Article
- Comparison of methods of extracting Salmonella enterica serovar Enteritidis DNA from environmental substrates and quantification of organisms by using a general internal procedural control.Applied and environmental microbiology · 2006Article
- Comparative analysis of the Schleicher and Schuell IsoCode Stix DNA isolation device and the Qiagen QIAamp DNA Mini Kit.Journal of clinical microbiology · 2004Article
- Real-time Fluorescent PCR Techniques to Study Microbial-Host Interactions.Methods in microbiology · 2004Review
- Detection of Yersinia pestis in sputum by real-time PCR.Journal of clinical microbiology · 2003Article
- Real-time PCR assay to detect smallpox virus.Journal of clinical microbiology · 2003Article
- Development of a real-time fluorescence PCR assay for rapid detection of the diphtheria toxin gene.Journal of clinical microbiology · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 5' nuclease PCR assay uses a fluorescently labeled oligonucleotide probe (TaqMan) to rapidly detect and quantitate DNA templates in clinical samples. We developed a 5' nuclease PCR assay targeting the plasminogen activator gene (pla) of Yersinia pestis. The assay is species specific, with a detection threshold of 2.1 x 10(5) copies of the pla target or 1.6 pg of total cell DNA. The assay detected Y. pestis in experimentally infected Xenopsylla cheopis fleas and in experimentally infected monkey blood and oropharyngeal swabs. The TaqMan assay is simple to perform and rapid and shows promise as a future field-adaptable technique.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.