ArticleJournal of clinical microbiology1998
Application of competitive PCR to cerebrospinal fluid samples from patients with herpes simplex encephalitis.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed.
- Epidemiology of Herpes Simplex and Varicella Zoster Virus-Associated Central Nervous System Infections in Western Greece: A Five-Year Retrospective Analysis.Pathogens (Basel, Switzerland) · 2025Article
- Comment on Cuesta et al. An Assessment of a New Rapid Multiplex PCR Assay for the Diagnosis of Meningoencephalitis.Diagnostics (Basel, Switzerland) · 2024Article
- Rapid-Onset Temporal Encephalitis With Negative Cerebrospinal Fluid Polymerase Chain Reaction Testing.Cureus · 2023Article
- Neuro-Axonal Damage and Alteration of Blood-Brain Barrier Integrity in COVID-19 Patients.Cells · 2022Article
- Relapse of Herpes Simplex Encephalitis by Epilepsy Surgery 35 Years after the First Infection: A Case Report and Literature Review.NMC case report journal · 2021Article
- Evaluation of multiplex real-time polymerase chain reaction for the detection of herpes simplex virus-1 and 2 and varicella-zoster virus in corneal cells from normal subjects and patients with keratitis in India.Indian journal of ophthalmology · 2019Article
- Suspected Herpes Simplex Encephalitis with Low Copy Number DNA.Indian journal of pediatrics · 2019Article
- Prevalence of herpes simplex virus-1 in hospitalized adult patients with clinical diagnosis of meningoencephalitis using real-time polymerase chain reaction: A single-center, cross-sectional study.Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2019Article
- Herpes Simplex Encephalitis: an Update.Current infectious disease reports · 2017Review
- HSV Encephalitis with Normal CSF - A Case Report with Review of Literature.Journal of clinical and diagnostic research : JCDR · 2015Article
- Fifteen minute consultation: managing neonatal and childhood herpes encephalitis.Archives of disease in childhood. Education and practice edition · 2015Review
- Diagnosing of herpes simplex virus infections in suspected patients using real-time PCR.Jundishapur journal of microbiology · 2015Article
- Clinical Applications of Quantitative Real-Time PCR in Virology.Methods in microbiology · 2015Article
- Light microscopy, culture, molecular, and serologic methods for detection of herpes simplex virus.Journal of clinical microbiology · 2014Review
- Impact of Herpes simplex virus load and red blood cells in cerebrospinal fluid upon herpes simplex meningo-encephalitis outcome.BMC infectious diseases · 2012Article
- Herpes simplex encephalitis with two false-negative cerebrospinal fluid PCR tests and review of negative PCR results in the clinical setting.Case reports in neurology · 2011Article
- Neonatal herpes simplex virus infections: where are we now?Advances in experimental medicine and biology · 2011Review
- Prognostic value of intrathecal antibody production and DNA viral load in cerebrospinal fluid of patients with herpes simplex encephalitis.Journal of neurology · 2009Article
- Persistence of herpes simplex virus DNA in cerebrospinal fluid of neonates with herpes simplex virus encephalitis.Journal of perinatology : official journal of the California Perinatal Association · 2009Article
- Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The purpose of the present study was to determine if the quantity of herpes simplex virus (HSV) DNA in the cerebrospinal fluid (CSF) of patients with herpes encephalitis would be useful in establishing the prognosis of the disease and to determine the effect of antiviral therapy on the clearance of viral DNA from the CSF. Quantitation of HSV DNA was done by constructing an internal standard (IS) from the glycoprotein B amplicon which had a 25-bp deletion between primer annealing sites. Each CSF specimen was coamplified with the IS and the ratio of the amount of HSV/amount of IS was compared to the ratios on a standard curve constructed with the same IS plus known amounts of HSV DNA. CSF specimens were available from 16 patients who were treated with intravenous acyclovir, and the amount of HSV DNA ranged from < 25 to 18,000 copies per microliter in CSF obtained before or within 4 days of the initiation of acyclovir therapy. Patients with > 100 copies of HSV DNA per microliter were older, were found by computed tomography to have lesions, and had poorer outcomes than patients with < 100 copies. Follow-up CSF specimens were available from seven patients. In six of these seven patients, the HSV DNA levels decreased during therapy. One patient had a twofold increase in HSV DNA levels after 1 week of therapy and died on day 8. The application of this assay may be helpful in establishing the prognosis and in the monitoring of patients with herpes simplex encephalitis.
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