ArticlePlant physiology1998
Characterization of spermidine binding to solubilized plasma membrane proteins from zucchini hypocotyls
Article in Plant physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
10 citing papers in PubMed, 54 citations in OpenAlex.
- Programmed Cell Death Reversal: Polyamines, Effectors of the U-Turn from the Program of Death inInternational journal of molecular sciences · 2024Review
- Pre-harvest spray of GABA and spermine delays postharvest senescence and alleviates chilling injury of gerbera cut flowers during cold storage.Scientific reports · 2021Article
- Changes in free polyamine levels, expression of polyamine biosynthesis genes, and performance of rice cultivars under salt stress: a comparison with responses to drought.Frontiers in plant science · 2014Article
- Polyamines induce adaptive responses in water deficit stressed cucumber roots.Journal of plant research · 2014Article
- Cadaverine: a lysine catabolite involved in plant growth and development.Plant signaling & behavior · 2013Article
- Review
- Polyamines improve K+/Na+ homeostasis in barley seedlings by regulating root ion channel activities.Plant physiology · 2007Article
- Ectomycorrhizal fungal species and strains differ in their ability to produce free and conjugated polyamines.Mycorrhiza · 2003Article
- Spermidine-binding proteins. Purification and expression analysis in maize.Plant physiology · 2002Article
- Cadaverine as a signal of heat shock in plants.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sectionsArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work [14C]spermidine binding to total proteins solubilized from plasma membrane purified from zucchini (Cucurbita pepo L.) hypocotyls was investigated. Proteins were solubilized using octyl glucoside as a detergent. Specific polyamine binding was thermolabile, reversible, pH dependent with an optimum at pH 8.0, and had a Kd value of 5 &mgr;M, as determined by glass-fiber-filter assays. Sephadex G-25 M gel-filtration assays confirmed the presence of a spermidine-protein(s) complex with a specific binding activity. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis and native polyacrylamide gel electrophoresis of collected fractions having the highest specific spermidine-binding activity, several protein bands (113, 75, 66, and 44 kD) were identified. The specificity of spermidine binding was examined by gel-filtration competition experiments performed using other polyamines and compounds structurally related to spermidine. Partial purification on Sephadex G-200 led to the identification of 66- and 44-kD protein bands, which may represent the putative spermidine-binding protein(s) on the plasmalemma.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.