ArticlePlant physiology1998
Dehydration-stress-regulated transgene expression in stably transformed rice plants
Article in Plant physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 71 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Comprehensive Genome-Wide Analysis and Expression Pattern Profiling of theInternational journal of molecular sciences · 2022Article
- Overexpression of AtDREB1D transcription factor improves drought tolerance in soybean.Molecular biology reports · 2014Article
- Comparative functional analysis of six drought-responsive promoters in transgenic rice.Planta · 2014Article
- Article
- Stress-inducible expression of barley Hva1 gene in transgenic mulberry displays enhanced tolerance against drought, salinity and cold stress.Transgenic research · 2012Article
- Analysis of transcriptional and upstream regulatory sequence activity of two environmental stress-inducible genes, NBS-Str1 and BLEC-Str8, of rice.Transgenic research · 2012Article
- Inducibility of three salinity/abscisic acid-regulated promoters in transgenic rice with gusA reporter gene.Plant cell reports · 2011Article
- Article
- Identification and expression profiling analysis of TIFY family genes involved in stress and phytohormone responses in rice.Plant molecular biology · 2009Article
- Comparative functional analysis of three abiotic stress-inducible promoters in transgenic rice.Transgenic research · 2009Article
- Over-expression of a LEA gene in rice improves drought resistance under the field conditions.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2007Article
- Article
- Overexpression of barley hva1 gene in creeping bentgrass for improving drought tolerance.Plant cell reports · 2007Article
- Computational prediction and experimental verification of HVA1-like abscisic acid responsive promoters in rice (Oryza sativa).Plant molecular biology · 2006Article
- Functional definition of ABA-response complexes: the promoter units necessary and sufficient for ABA induction of gene expression in barley ( Hordeum vulgare L.).Plant molecular biology · 2004Article
- Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To confer abscisic acid (ABA) and/or stress-inducible gene expression, an ABA-response complex (ABRC1) from the barley (Hordeum vulgare L.) HVA22 gene was fused to four different lengths of the 5' region from the rice (Oryza sativa L.) Act1 gene. Transient assay of beta-glucuronidase (GUS) activity in barley aleurone cells shows that, coupled with ABRC1, the shortest minimal promoter (Act1-100P) gives both the greatest induction and the highest level of absolute activity following ABA treatment. Two plasmids with one or four copies of ABRC1 combined with the same Act1-100P and HVA22(I) of barley HVA22 were constructed and used for stable expression of uidA in transgenic rice plants. Three Southern blot-positive lines with the correct hybridization pattern for each construct were obtained. Northern analysis indicated that uidA expression is induced by ABA, water-deficit, and NaCl treatments. GUS activity assays in the transgenic plants confirmed that the induction of GUS activity varies from 3- to 8-fold with different treatments or in different rice tissues, and that transgenic rice plants harboring four copies of ABRC1 show 50% to 200% higher absolute GUS activity both before and after treatments than those with one copy of ABRC1.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.