ArticleThe Journal of physiology1998
Regulation of Ca2+-dependent Cl- conductance in a human colonic epithelial cell line (T84): cross-talk between Ins(3,4,5,6)P4 and protein phosphatases.
Article in The Journal of physiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 55 citations in OpenAlex.
- Review
- Calmodulin-Dependent Regulation of Overexpressed but Not Endogenous TMEM16A Expressed in Airway Epithelial Cells.Membranes · 2021Article
- Control of TMEM16A by INO-4995 and other inositolphosphates.British journal of pharmacology · 2013Article
- Defining signal transduction by inositol phosphates.Sub-cellular biochemistry · 2012Review
- Molecular basis for the integration of inositol phosphate signaling pathways via human ITPK1.Advances in enzyme regulation · 2009Review
- Mechanism of the inhibition of Ca2+-activated Cl- currents by phosphorylation in pulmonary arterial smooth muscle cells.The Journal of general physiology · 2006Article
- Annexin A4 self-association modulates general membrane protein mobility in living cells.Molecular biology of the cell · 2006Article
- Inositol polyphosphate derivative inhibits Na+ transport and improves fluid dynamics in cystic fibrosis airway epithelia.American journal of physiology. Cell physiology · 2005Article
- Identification of an N-terminal amino acid of the CLC-3 chloride channel critical in phosphorylation-dependent activation of a CaMKII-activated chloride current.The Journal of physiology · 2004Article
- How versatile are inositol phosphate kinases?The Biochemical journal · 2004Review
- Article
- The Ca-activated Cl channel and its control in rat olfactory receptor neurons.The Journal of general physiology · 2003Article
- State and spectral properties of chloride oscillations in pollen.Biophysical journal · 2003Article
- Cloning and expression of a cDNA encoding human inositol 1,4,5-trisphosphate 3-kinase C.The Biochemical journal · 2000Article
- Multitasking in signal transduction by a promiscuous human Ins(3,4,5,6)P(4) 1-kinase/Ins(1,3,4)P(3) 5/6-kinase.The Biochemical journal · 2000Article
- Syntaxin 1A is expressed in airway epithelial cells, where it modulates CFTR Cl(-) currents.The Journal of clinical investigation · 2000Article
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Authors and funding
7 authors at 6 institutions in 2 countries.
Funding
Abstract
1. We have studied the regulation of whole-cell chloride current in T84 colonic epithelial cells by inositol 3,4,5,6-tetrakisphosphate (Ins(3,4,5,6)P4). New information was obtained using (a) microcystin and okadaic acid to inhibit serine/threonine protein phosphatases, and (b) a novel functional tetrakisphosphate analogue, 1, 2-bisdeoxy-1,2-bisfluoro-Ins(3,4,5,6)P4 (i.e. F2-Ins(3,4,5,6)P4). 2. Calmodulin-dependent protein kinase II (CaMKII) increased chloride current 20-fold. This current (ICl,CaMK) continued for 7 +/- 1.2 min before its deactivation, or running down, by approximately 60 %. This run-down was prevented by okadaic acid, whereupon ICl,CaMK remained near its maximum value for >= 14.3 +/- 0.6 min. 3. F2-Ins(3, 4,5,6)P4 inhibited ICl,CaMK (IC50 = 100 microM) stereo-specifically, since its enantiomer, F2-Ins(1,4,5,6)P4 had no effect at >= 500 microM. Dose-response data (Hill coefficient = 1.3) showed that F2-Ins(3,4,5,6)P4 imitated only the non-co-operative phase of inhibition by Ins(3,4,5,6)P4, and not the co-operative phase. 4. Ins(3,4,5,6)P4 was prevented from blocking ICl,CaMK by okadaic acid (IC50 = 1.5 nM) and microcystin (IC50 = 0.15 nM); these data lead to the novel conclusion that, in situ, protein phosphatase activity is essential for Ins(3,4,5,6)P4 to function. The IC50 values indicate that more than one species of phosphatase was required. One of these may be PP1, since F2-Ins(3,4,5,6)P4-dependent current blocking was inhibited by okadaic acid and microcystin with IC50 values of 70 nM and 0.15 nM, respectively.
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