ArticleThe Journal of cell biology1998
Functional specialization of stable and dynamic microtubules in protein traffic in WIF-B cells.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 52 citations in OpenAlex.
- Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy.Journal of visualized experiments : JoVE · 2022Article
- Differential regulation of single microtubules and bundles by a three-protein module.Nature chemical biology · 2021Article
- Structural and functional hepatocyte polarity and liver disease.Journal of hepatology · 2015Review
- The Altered Hepatic Tubulin Code in Alcoholic Liver Disease.Biomolecules · 2015Review
- Alcohol-induced defects in hepatic transcytosis may be explained by impaired dynein function.Molecular and cellular biochemistry · 2014Article
- Hepatocyte polarity.Comprehensive Physiology · 2013Review
- Starvation-induced hyperacetylation of tubulin is required for the stimulation of autophagy by nutrient deprivation.The Journal of biological chemistry · 2010Article
- Alcohol consumption impairs hepatic protein trafficking: mechanisms and consequences.Genes & nutrition · 2010Article
- Alcohol-induced alterations of the hepatocyte cytoskeleton.World journal of gastroenterology · 2010Review
- Alcohol-induced protein hyperacetylation: mechanisms and consequences.World journal of gastroenterology · 2009Review
- Microtubule acetylation and stability may explain alcohol-induced alterations in hepatic protein trafficking.Hepatology (Baltimore, Md.) · 2008Article
- A sesquiterpene quinone, 5-Epi-smenospongine, promotes TNF-α production in LPS-stimulated RAW 264.7 Cells.Marine drugs · 2007Article
- Microtubules regulate disassembly of epithelial apical junctions.BMC cell biology · 2006Article
- The Golgi complex is a microtubule-organizing organelle.Molecular biology of the cell · 2001Article
- Article
- PAT1, a microtubule-interacting protein, recognizes the basolateral sorting signal of amyloid precursor protein.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We found that the magnesium salt of ilimaquinone, named 201-F, specifically disassembled dynamically unstable microtubules in fibroblasts and various epithelial cell lines. Unlike classical tubulin- interacting drugs such as nocodazole or colchicine which affect all classes of microtubules, 201-F did not depolymerize stable microtubules. In WIF-B-polarized hepatic cells, 201-F disrupted the Golgi complex and inhibited albumin and alpha1-antitrypsin secretion to the same extent as nocodazole. By contrast, 201-F did not impair the transport of membrane proteins to the basolateral surface, which was only affected by the total disassembly of cellular microtubules. Transcytosis of two apical membrane proteins-the alkaline phosphodiesterase B10 and dipeptidyl peptidase IV-was affected to the same extent by 201-F and nocodazole. Taken together, these results indicate that only dynamically unstable microtubules are involved in the transport of secretory proteins to the plasma membrane, and in the transcytosis of membrane proteins to the apical surface. By contrast, stable microtubules, which are not functionally affected by 201-F treatment, are involved in the transport of membrane proteins to the basolateral surface. By specifically disassembling highly dynamic microtubules, 201-F is an invaluable tool with which to study the functional specialization of stable and dynamic microtubules in living cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.