ArticleMolecular biology of the cell1998
Actin filaments and microtubules are involved in different membrane traffic pathways that transport sphingolipids to the apical surface of polarized HepG2 cells.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 60 citations in OpenAlex.
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- Intersectin-1 interacts with the golgin GCC88 to couple the actin network and Golgi architecture.Molecular biology of the cell · 2019Article
- Spermine oxidase promotes bile canalicular lumen formation through acrolein production.Scientific reports · 2017Article
- Study of Ethanol-Induced Golgi Disorganization Reveals the Potential Mechanism of Alcohol-Impaired N-Glycosylation.Alcoholism, clinical and experimental research · 2016Article
- Structural and functional hepatocyte polarity and liver disease.Journal of hepatology · 2015Review
- Contribution of mesenchymal proliferation in tooth root morphogenesis.Journal of dental research · 2014Article
- Hepatocyte polarity.Comprehensive Physiology · 2013Review
- Hepatocytes traffic and export hepatitis B virus basolaterally by polarity-dependent mechanisms.Journal of virology · 2011Article
- Structure and function of ABCG2-rich extracellular vesicles mediating multidrug resistance.PloS one · 2011Article
- Effect of Cytoskeletal Disruption on Mechanotransduction of Hydrostatic Pressure by C3H10T1/2 Murine Fibroblasts.The open orthopaedics journal · 2008Article
- Binding of CAP70 to inducible nitric oxide synthase and implications for the vectorial release of nitric oxide in polarized cells.Molecular biology of the cell · 2007Article
- Rho kinase, myosin-II, and p42/44 MAPK control extracellular matrix-mediated apical bile canalicular lumen morphogenesis in HepG2 cells.Molecular biology of the cell · 2006Article
- Uptake and transport of PEG-graft-trimethyl-chitosan copolymer-insulin nanocomplexes by epithelial cells.Pharmaceutical research · 2005Article
- Differential effects of actin cytoskeleton dynamics on equine infectious anemia virus particle production.Journal of virology · 2004Article
- MAL2, a novel raft protein of the MAL family, is an essential component of the machinery for transcytosis in hepatoma HepG2 cells.The Journal of cell biology · 2002Article
- Article
- Cytokeratins as targets for bile acid-induced toxicity.The American journal of pathology · 2002Article
- Polarized sphingolipid transport from the subapical compartment: evidence for distinct sphingolipid domains.Molecular biology of the cell · 1999Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In polarized HepG2 hepatoma cells, sphingolipids are transported to the apical, bile canalicular membrane by two different transport routes, as revealed with fluorescently tagged sphingolipid analogs. One route involves direct, transcytosis-independent transport of Golgi-derived glucosylceramide and sphingomyelin, whereas the other involves basolateral to apical transcytosis of both sphingolipids. We show that these distinct routes display a different sensitivity toward nocodazole and cytochalasin D, implying a specific transport dependence on either microtubules or actin filaments, respectively. Thus, nocodazole strongly inhibited the direct route, whereas sphingolipid transport by transcytosis was hardly affected. Moreover, nocodazole blocked "hyperpolarization," i.e., the enlargement of the apical membrane surface, which is induced by treating cells with dibutyryl-cAMP. By contrast, the transcytotic route but not the direct route was inhibited by cytochalasin D. The actin-dependent step during transcytotic lipid transport probably occurs at an early endocytic event at the basolateral plasma membrane, because total lipid uptake and fluid phase endocytosis of horseradish peroxidase from this membrane were inhibited by cytochalasin D as well. In summary, the results show that the two sphingolipid transport pathways to the apical membrane must have a different requirement for cytoskeletal elements.
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