ArticleMolecular biology of the cell1998
Multiple domains of fission yeast Cdc19p (MCM2) are required for its association with the core MCM complex.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 52 citations in OpenAlex.
- Identification of MCM2-Interacting Proteins Associated with Replication Initiation Using APEX2-Based Proximity Labeling Technology.International journal of molecular sciences · 2025Article
- Article
- Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4.G3 (Bethesda, Md.) · 2016Article
- Expression, purification and biochemical characterization of Schizosaccharomyces pombe Mcm4, 6 and 7.BMC biochemistry · 2013Article
- Mcm10 plays an essential role in origin DNA unwinding after loading of the CMG components.The EMBO journal · 2012Article
- Schizosaccharomyces pombe minichromosome maintenance-binding protein (MCM-BP) antagonizes MCM helicase.The Journal of biological chemistry · 2011Article
- Auxin-inducible protein depletion system in fission yeast.BMC cell biology · 2011Article
- Hsk1 kinase and Cdc45 regulate replication stress-induced checkpoint responses in fission yeast.Cell cycle (Georgetown, Tex.) · 2010Article
- The Mcm complex: unwinding the mechanism of a replicative helicase.Microbiology and molecular biology reviews : MMBR · 2009Review
- Dynamic localization of the DNA replication proteins MCM5 and MCM7 in plants.Plant physiology · 2009Article
- Article
- Minichromosome maintenance proteins interact with checkpoint and recombination proteins to promote s-phase genome stability.Molecular and cellular biology · 2008Article
- Identification and characterization of a novel component of the human minichromosome maintenance complex.Molecular and cellular biology · 2007Article
- Article
- Schizosaccharomyces pombe replication protein Cdc45/Sna41 requires Hsk1/Cdc7 and Rad4/Cut5 for chromatin binding.Chromosoma · 2004Article
- A novel intermediate in initiation complex assembly for fission yeast DNA replication.Molecular biology of the cell · 2004Article
- Eukaryotic MCM proteins: beyond replication initiation.Microbiology and molecular biology reviews : MMBR · 2004Review
- Ibp1p, a novel Cdc25-related phosphatase, suppresses Schizosaccharomyces pombe hsk1 ( cdc7).Current genetics · 2003Article
- Article
- Characterization of Schizosaccharomyces pombe mcm7(+) and cdc23(+) (MCM10) and interactions with replication checkpoints.Genetics · 2001Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The members of the MCM protein family are essential eukaryotic DNA replication factors that form a six-member protein complex. In this study, we use antibodies to four MCM proteins to investigate the structure of and requirements for the formation of fission yeast MCM complexes in vivo, with particular regard to Cdc19p (MCM2). Gel filtration analysis shows that the MCM protein complexes are unstable and can be broken down to subcomplexes. Using coimmunoprecipitation, we find that Mis5p (MCM6) and Cdc21p (MCM4) are tightly associated with one another in a core complex with which Cdc19p loosely associates. Assembly of Cdc19p with the core depends upon Cdc21p. Interestingly, there is no obvious change in Cdc19p-containing MCM complexes through the cell cycle. Using a panel of Cdc19p mutants, we find that multiple domains of Cdc19p are required for MCM binding. These studies indicate that MCM complexes in fission yeast have distinct substructures, which may be relevant for function.
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