ArticleMolecular biology of the cell1998
Identification of novel temperature-sensitive lethal alleles in essential beta-tubulin and nonessential alpha 2-tubulin genes as fission yeast polarity mutants.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- The Mis6 inner kinetochore subcomplex maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosis.Communications biology · 2022Article
- Microtubules in Microorganisms: How Tubulin Isotypes Contribute to Diverse Cytoskeletal Functions.Frontiers in cell and developmental biology · 2022Review
- A visual atlas of meiotic protein dynamics in living fission yeast.Open biology · 2021Article
- Escape from mitotic catastrophe by actin-dependent nuclear displacement in fission yeast.iScience · 2021Article
- Local and global Cdc42 guanine nucleotide exchange factors for fission yeast cell polarity are coordinated by microtubules and the Tea1-Tea4-Pom1 axis.Journal of cell science · 2018Article
- The hairpin region of Ndc80 is important for the kinetochore recruitment of Mph1/MPS1 in fission yeast.Cell cycle (Georgetown, Tex.) · 2016Article
- Optimization of the analogue-sensitive Cdc2/Cdk1 mutant by in vivo selection eliminates physiological limitations to its use in cell cycle analysis.Open biology · 2014Article
- Article
- Mzt1/Tam4, a fission yeast MOZART1 homologue, is an essential component of the γ-tubulin complex and directly interacts with GCP3(Alp6).Molecular biology of the cell · 2013Article
- Microtubules and Alp7-Alp14 (TACC-TOG) reposition chromosomes before meiotic segregation.Nature cell biology · 2013Article
- The dual role of fission yeast Tbc1/cofactor C orchestrates microtubule homeostasis in tubulin folding and acts as a GAP for GTPase Alp41/Arl2.Molecular biology of the cell · 2013Article
- The internal loop of fission yeast Ndc80 binds Alp7/TACC-Alp14/TOG and ensures proper chromosome attachment.Molecular biology of the cell · 2013Article
- Schizosaccharomyces pombe minichromosome maintenance-binding protein (MCM-BP) antagonizes MCM helicase.The Journal of biological chemistry · 2011Article
- Fission yeast cells undergo nuclear division in the absence of spindle microtubules.PLoS biology · 2010Article
- Shaping fission yeast with microtubules.Cold Spring Harbor perspectives in biology · 2009Review
- Secondary mutations correct fitness defects in Toxoplasma gondii with dinitroaniline resistance mutations.Genetics · 2008Article
- Pom1 DYRK regulates localization of the Rga4 GAP to ensure bipolar activation of Cdc42 in fission yeast.Current biology : CB · 2008Article
- Chromosome segregation in fission yeast with mutations in the tubulin folding cofactor D.Current genetics · 2006Article
- Cytoplasmic microtubule organization in fission yeast.Yeast (Chichester, England) · 2006Review
- The fission yeast transforming acidic coiled coil-related protein Mia1p/Alp7p is required for formation and maintenance of persistent microtubule-organizing centers at the nuclear envelope.Molecular biology of the cell · 2006Article
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5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have screened for temperature-sensitive (ts) fission yeast mutants with altered polarity (alp1-15). Genetic analysis indicates that alp2 is allelic to atb2 (one of two alpha-tubulin genes) and alp12 to nda3 (the single beta-tubulin gene). atb2(+) is nonessential, and the ts atb2 mutations we have isolated are dominant as expected. We sequenced two alleles of ts atb2 and one allele of ts nda3. In the ts atb2 mutants, the mutated residues (G246D and C356Y) are found at the longitudinal interface between alpha/beta-heterodimers, whereas in ts nda3 the mutated residue (Y422H) is situated in the domain located on the outer surface of the microtubule. The ts nda3 mutant is highly sensitive to altered gene dosage of atb2(+); overexpression of atb2(+) lowers the restrictive temperature, and, conversely, deletion rescues ts. Phenotypic analysis shows that contrary to undergoing mitotic arrest with high viability via the spindle assembly checkpoint as expected, ts nda3 mutants execute cytokinesis and septation and lose viability. Therefore, it appears that the ts nda3 mutant becomes temperature lethal because of irreversible progression through the cell cycle in the absence of activating the spindle assembly checkpoint pathway.
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