ArticleMolecular biology of the cell1998
The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 117 citations in OpenAlex.
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- Contribution of the yeast bi-chaperone system in the restoration of the RNA helicase Ded1 and translational activity under severe ethanol stress.The Journal of biological chemistry · 2023Article
- Actin- and microtubule-based motors contribute to clathrin-independent endocytosis in yeast.Molecular biology of the cell · 2023Article
- Loss of Num1-mediated cortical dynein anchoring negatively impacts respiratory growth.Journal of cell science · 2022Article
- Acquired Resistance to Severe Ethanol Stress in Saccharomyces cerevisiae Protein Quality Control.Applied and environmental microbiology · 2021Article
- Nuclear movement in fungi.Seminars in cell & developmental biology · 2018Review
- Prioritized Expression of BTN2 of Saccharomyces cerevisiae under Pronounced Translation Repression Induced by Severe Ethanol Stress.Frontiers in microbiology · 2016Article
- Prioritized Expression of BDH2 under Bulk Translational Repression and Its Contribution to Tolerance to Severe Vanillin Stress in Saccharomyces cerevisiae.Frontiers in microbiology · 2016Article
- Asymmetry of the budding yeast Tem1 GTPase at spindle poles is required for spindle positioning but not for mitotic exit.PLoS genetics · 2015Article
- The ADH7 Promoter of Saccharomyces cerevisiae is Vanillin-Inducible and Enables mRNA Translation Under Severe Vanillin Stress.Frontiers in microbiology · 2015Article
- The RNA-binding protein Whi3 is a key regulator of developmental signaling and ploidy in Saccharomyces cerevisiae.Genetics · 2013Article
- Biomass conversion inhibitors furfural and 5-hydroxymethylfurfural induce formation of messenger RNP granules and attenuate translation activity in Saccharomyces cerevisiae.Applied and environmental microbiology · 2013Article
- Evolution of the eukaryotic dynactin complex, the activator of cytoplasmic dynein.BMC evolutionary biology · 2012Article
- Mitotic spindle form and function.Genetics · 2012Article
- A novel patch assembly domain in Num1 mediates dynein anchoring at the cortex during spindle positioning.The Journal of cell biology · 2012Article
- Functional interaction between dynein light chain and intermediate chain is required for mitotic spindle positioning.Molecular biology of the cell · 2011Article
- Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150(Glued) and DNC-2/dynamitin.Genes to cells : devoted to molecular & cellular mechanisms · 2010Article
- Genome-wide scoring of positive and negative epistasis through decomposition of quantitative genetic interaction fitness matrices.PloS one · 2010Article
- Function of dynein in budding yeast: mitotic spindle positioning in a polarized cell.Cell motility and the cytoskeleton · 2009Review
- Arp11 affects dynein-dynactin interaction and is essential for dynein function in Aspergillus nidulans.Traffic (Copenhagen, Denmark) · 2008Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although vertebrate cytoplasmic dynein can move to the minus ends of microtubules in vitro, its ability to translocate purified vesicles on microtubules depends on the presence of an accessory complex known as dynactin. We have cloned and characterized a novel gene, NIP100, which encodes the yeast homologue of the vertebrate dynactin complex protein p150(glued). Like strains lacking the cytoplasmic dynein heavy chain Dyn1p or the centractin homologue Act5p, nip100Delta strains are viable but undergo a significant number of failed mitoses in which the mitotic spindle does not properly partition into the daughter cell. Analysis of spindle dynamics by time-lapse digital microscopy indicates that the precise role of Nip100p during anaphase is to promote the translocation of the partially elongated mitotic spindle through the bud neck. Consistent with the presence of a true dynactin complex in yeast, Nip100p exists in a stable complex with Act5p as well as Jnm1p, another protein required for proper spindle partitioning during anaphase. Moreover, genetic depletion experiments indicate that the binding of Nip100p to Act5p is dependent on the presence of Jnm1p. Finally, we find that a fusion of Nip100p to the green fluorescent protein localizes to the spindle poles throughout the cell cycle. Taken together, these results suggest that the yeast dynactin complex and cytoplasmic dynein together define a physiological pathway that is responsible for spindle translocation late in anaphase.
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