ArticleJournal of virology1998
Binding of the human immunodeficiency virus type 1 Gag protein to the viral RNA encapsidation signal in the yeast three-hybrid system.
Article in Journal of virology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Initiation of HIV-1 Gag lattice assembly is required for recognition of the viral genome packaging signal.eLife · 2023Article
- Human Retrovirus Genomic RNA Packaging.Viruses · 2022Review
- Inferring Protein Function in an Emerging Virus: Detection of the Nucleoprotein in Tilapia Lake Virus.Journal of virology · 2022Article
- Large ribosomal protein 4 increases efficiency of viral recoding sequences.Journal of virology · 2012Article
- Transduction of human immunodeficiency virus type 1 vectors lacking encapsidation and dimerization signals.Journal of virology · 2007Article
- Development of a cell-based assay probing the specific interaction between the human immunodeficiency virus type 1 nucleocapsid and psi RNA in vivo.Journal of virology · 2007Article
- Single point mutations in the zinc finger motifs of the human immunodeficiency virus type 1 nucleocapsid alter RNA binding specificities of the gag protein and enhance packaging and infectivity.Journal of virology · 2005Article
- Dual role of the lymphocytic choriomeningitis virus intergenic region in transcription termination and virus propagation.Journal of virology · 2005Article
- The conserved carboxy terminus of the capsid domain of human immunodeficiency virus type 1 gag protein is important for virion assembly and release.Journal of virology · 2004Article
- RNA sequences in the Moloney murine leukemia virus genome bound by the Gag precursor protein in the yeast three-hybrid system.Journal of virology · 2004Article
- The leader of human immunodeficiency virus type 1 genomic RNA harbors an internal ribosome entry segment that is active during the G2/M phase of the cell cycle.Journal of virology · 2003Article
- Interaction of zyxin, a focal adhesion protein, with the e6 protein from human papillomavirus type 6 results in its nuclear translocation.Journal of virology · 2001Article
- Identification of residues of the Moloney murine leukemia virus nucleocapsid critical for viral DNA synthesis in vivo.Journal of virology · 2001Article
- Gps2, a protein partner for human papillomavirus E6 proteins.Journal of virology · 2001Article
- The carboxy-terminal fragment of nucleolin interacts with the nucleocapsid domain of retroviral gag proteins and inhibits virion assembly.Journal of virology · 2000Article
- Assembly of retrovirus capsid-nucleocapsid proteins in the presence of membranes or RNA.Journal of virology · 2000Article
- Interaction of the cauliflower mosaic virus coat protein with the pregenomic RNA leader.Journal of virology · 2000Article
- The gag domains required for avian retroviral RNA encapsidation determined by using two independent assays.Journal of virology · 1999Article
- Identification of RNAs that bind to a specific protein using the yeast three-hybrid system.RNA (New York, N.Y.) · 1999Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have used the yeast three-hybrid system (D. J. SenGupta, B. Zhang, B. Kraemer, P. Pochart, S. Fields, and M. Wickens, Proc. Natl. Acad. Sci. USA 93:8496-8501, 1996) to study binding of the human immunodeficiency virus type 1 (HIV-1) Gag protein to the HIV-1 RNA encapsidation signal (HIVPsi). Interaction of these elements results in the activation of a reporter gene in the yeast Saccharomyces cerevisiae. Using this system, we have shown that the HIV-1 Gag protein binds specifically to a 139-nucleotide fragment of the HIVPsi signal containing four stem-loop structures. Mutations in either the Gag protein or the encapsidation signal that have been shown previously to impair this interaction reduced the activation of the reporter gene. Interestingly, the nucleocapsid portion of Gag retained the RNA binding activity but lost its specificity compared to the full-length Gag. These results demonstrate the utility of this system and suggest that a variety of genetic analyses could be performed to study Gag-encapsidation signal interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.