ArticleJournal of virology1998
In vivo Ty1 reverse transcription can generate replication intermediates with untidy ends.
Article in Journal of virology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Quadruplex-Forming Motif Inserted into 3'UTR ofBiology · 2021Article
- Genetic Characterization of Three Distinct Mechanisms Supporting RNA-Driven DNA Repair and Modification Reveals Major Role of DNA Polymerase ζ.Molecular cell · 2020Article
- Article
- Determinants of Genomic RNA Encapsidation in the Saccharomyces cerevisiae Long Terminal Repeat Retrotransposons Ty1 and Ty3.Viruses · 2016Review
- The Ty1 LTR-retrotransposon of budding yeast,Microbiology spectrum · 2015Article
- Ty1 integrase overexpression leads to integration of non-Ty1 DNA fragments into the genome of Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2010Review
- Retrotransposon suicide: formation of Ty1 circles and autointegration via a central DNA flap.Journal of virology · 2006Article
- A new rapid amplification of cDNA ends method for extremely guanine plus cytosine-rich genes.Analytical biochemistry · 2006Article
- The self primer of the long terminal repeat retrotransposon Tf1 is not removed during reverse transcription.Journal of virology · 2006Article
- Specific recognition and cleavage of the plus-strand primer by reverse transcriptase.Journal of virology · 2005Article
- An evaluation of detection methods for large lariat RNAs.RNA (New York, N.Y.) · 2005Article
- Foamy virus integration.Journal of virology · 2004Article
- A nucleocapsid functionality contained within the amino terminus of the Ty1 protease that is distinct and separable from proteolytic activity.Journal of virology · 2002Article
- DNA synthesis fidelity by the reverse transcriptase of the yeast retrotransposon Ty1.Nucleic acids research · 2001Article
- DNA integration by Ty integrase in yku70 mutant Saccharomyces cerevisiae cells.Molecular and cellular biology · 2000Article
- Deletion of a short, untranslated region adjacent to the polypurine tract in Moloney murine leukemia virus leads to formation of aberrant 5' plus-strand DNA ends in vivo.Journal of virology · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Ty1 retrotransposition, like retroviral replication, is a complex series of events requiring reverse transcription of an RNA intermediate, RNA-primed minus- and plus-strand DNA synthesis, multiple strand transfers, and precise cleavages of the template and primers by RNase H. In this report, we examine the structure of in vivo Ty1 replication intermediates, specifically with regard to the behavior of reverse transcriptase upon reaching template ends and to the precision with which RNase H might generate these ends. While the expected 3' termini were always identified, terminal nontemplated bases were also observed at all of the RNA and DNA template ends examined. Nontemplated A residues were most common at all 3' ends, although C residues were preferentially added to minus-strand termini paused at the 5' end of capped Ty1 RNA. In addition, we observed that RNase H removal of the tRNA primer and of the polypurine tract was not always precise or efficient. Finally, we noted numerous instances of Ty1 reverse transcriptase transferring from normal Ty1 template ends to various tRNA templates, with continued synthesis to specific modified bases. A similar pattern was obtained for Ty2, indicating that template ends offer unique opportunities for these two related reverse transcriptases to generate errors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.