ArticleProceedings of the National Academy of Sciences of the United States of America1998
Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 133 citations in OpenAlex.
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- SETD2-mediated H3K14 trimethylation promotes ATR activation and stalled replication fork restart in response to DNA replication stress.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Peperomin E and its orally bioavailable analog induce oxidative stress-mediated apoptosis of acute myeloid leukemia progenitor cells by targeting thioredoxin reductase.Redox biology · 2019Article
- Roles of SETD2 in Leukemia-Transcription, DNA-Damage, and Beyond.International journal of molecular sciences · 2019Review
- GMF as an Actin Network Remodeling Factor.Trends in cell biology · 2018Review
- Article
- SETD2: an epigenetic modifier with tumor suppressor functionality.Oncotarget · 2016Review
- Preparation of Proper Immunogen by Cloning and Stable Expression of cDNA coding for Human Hematopoietic Stem Cell Marker CD34 in NIH-3T3 Mouse Fibroblast Cell Line.Advanced pharmaceutical bulletin · 2015Article
- Ubiquitous expression of MAKORIN-2 in normal and malignant hematopoietic cells and its growth promoting activity.PloS one · 2014Article
- Clinical bioinformatics: a new emerging science.Journal of clinical bioinformatics · 2011Article
- Transcriptome study for early hematopoiesis--achievement, challenge and new opportunity.Journal of cellular physiology · 2010Article
- The transcriptome of human CD34+ hematopoietic stem-progenitor cells.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Analysis of phenotype-genotype connection: the story of dissecting disease pathogenesis in genomic era in China, and beyond.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2007Review
- Mitochondrial protein import and human health and disease.Biochimica et biophysica acta · 2007Review
- Glia maturation factor gamma (GMFG): a cytokine-responsive protein during hematopoietic lineage development and its functional genomics analysis.Genomics, proteomics & bioinformatics · 2006Article
Corrections and comments
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Authors and funding
17 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hematopoietic stem/progenitor cells (HSPCs) possess the potentials of self-renewal, proliferation, and differentiation toward different lineages of blood cells. These cells not only play a primordial role in hematopoietic development but also have important clinical application. Characterization of the gene expression profile in CD34(+) HSPCs may lead to a better understanding of the regulation of normal and pathological hematopoiesis. In the present work, genes expressed in human umbilical cord blood CD34(+) cells were catalogued by partially sequencing a large amount of cDNA clones [or expressed sequence tags (ESTs)] and analyzing these sequences with the tools of bioinformatics. Among 9,866 ESTs thus obtained, 4,697 (47.6%) showed identity to known genes in the GenBank database, 2, 603 (26.4%) matched to the ESTs previously deposited in a public domain database, 1,415 (14.3%) were previously undescribed ESTs, and the remaining 1,151 (11.7%) were mitochondrial DNA, ribosomal RNA, or repetitive (Alu or L1) sequences. Integration of ESTs of known genes generated a profile including 855 genes that could be divided into different categories according to their functions. Some (8.2%) of the genes in this profile were considered related to early hematopoiesis. The possible function of ESTs corresponding to so far unknown genes were approached by means of homology and functional motif searches. Moreover, attempts were made to generate libraries enriched for full-length cDNAs, to better explore the genes in HSPCs. Nearly 60% of the cDNA clones of mRNA under 2 kb in our libraries had 5' ends upstream of the first ATG codon of the ORF. With this satisfactory result, we have developed an efficient working system that allowed fast sequencing of 32 full-length cDNAs, 16 of them being mapped to the chromosomes with radiation hybrid panels. This work may lay a basis for the further research on the molecular network of hematopoietic regulation.
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