ArticleProceedings of the National Academy of Sciences of the United States of America1998
Localization of signal recognition particle RNA in the nucleolus of mammalian cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
74 citing papers in PubMed, 153 citations in OpenAlex.
- Signal recognition particle 14 binds to importin α in Plasmodium falciparum.Molecular biology reports · 2026Article
- Signaling to make human ribosomes: Connections between the cytoplasm and the nucleolus.Molecular cell · 2026Review
- Widespread mono- and oligoadenylation direct small noncoding RNA maturation versus degradation fates.The EMBO journal · 2026Article
- Anticancer drug development against ribosome synthesis and the nucleolus.Biochemical Society transactions · 2025Article
- The nucleolar phase of signal recognition particle assembly.Life science alliance · 2024Article
- The role of SRP9/SRP14 in regulating Alu RNA.RNA biology · 2024Review
- Beyond ribosome biogenesis: noncoding nucleolar RNAs in physiology and tumor biology.Nucleus (Austin, Tex.) · 2023Review
- The human cellular protein NoL12 is a specific partner of the HIV-1 nucleocapsid protein NCp7.Journal of virology · 2023Article
- An Intermittent Cytochemist.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
- The Nucleolus: In Genome Maintenance and Repair.International journal of molecular sciences · 2017Review
- A novel hypothesis for histone-to-protamine transition in Bos taurus spermatozoa.Reproduction (Cambridge, England) · 2017Article
- Novel Ribonuclease Activity Differs between Fibrillarins fromFrontiers in plant science · 2017Article
- Grabbing the genome by the NADs.Chromosoma · 2016Review
- Proteomic characterization of the nucleolar linker histone H1 interaction network.Journal of molecular biology · 2015Article
- Thoru Pederson: Spotting novel roles for the nucleolus.The Journal of cell biology · 2015Article
- Functional ultrastructure of the plant nucleolus.Protoplasma · 2014Review
- Stressing on the nucleolus in cardiovascular disease.Biochimica et biophysica acta · 2014Review
- Probing the stiffness of isolated nucleoli by atomic force microscopy.Histochemistry and cell biology · 2014Article
- Plasmodium falciparum signal recognition particle components and anti-parasitic effect of ivermectin in blocking nucleo-cytoplasmic shuttling of SRP.Cell death & disease · 2014Article
- RNA gymnastics in mammalian signal recognition particle assembly.RNA biology · 2014Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The signal recognition particle (SRP) of eukaryotic cells is a cytoplasmic ribonucleoprotein machine that arrests the translational elongation of nascent secretory and membrane proteins and facilitates their transport into the endoplasmic reticulum. The spatial pathway of SRP RNA processing and ribonucleoprotein assembly in the cell is not known. In the present investigation, microinjection of fluorescently tagged SRP RNA into the nucleus of mammalian cells was used to examine its intranuclear sites of localization. Microinjection of SRP RNA into the nuclei of normal rat kidney (NRK) epithelial cells maintained at 37 degreesC on the microscope stage resulted in a very rapid initial localization in nucleoli, followed by a progressive decline of nucleolar signal and an increase of fluorescence at discrete sites in the cytoplasm. Nuclear microinjection of a molecule corresponding to a major portion of the Alu domain of SRP RNA revealed a pattern of rapid nucleolar localization followed by cytoplasmic appearance of signal that was similar to the results obtained with full-length SRP RNA. In contrast, a molecule corresponding to the S domain of SRP RNA did not display nucleolar localization to the extent observed with full-length SRP RNA. An SRP RNA molecule lacking helix 6 of the S domain displayed normal nucleolar localization, whereas one lacking helix 8 of the S domain did not. These results, obtained by direct, real-time observation of fluorescent RNA molecules inside the nucleus of living mammalian cells, suggest that the processing of SRP RNA or its ribonucleoprotein assembly into the SRP involves a nucleolar phase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.