ArticleThe Journal of experimental medicine1998
Phase-variable expression of lipopolysaccharide contributes to the virulence of legionella pneumophila.
Article in The Journal of experimental medicine, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 70 citations in OpenAlex.
- 'Epidemiology ofMicrobial genomics · 2026Article
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- The unique Legionella longbeachae capsule favors intracellular replication and immune evasion.PLoS pathogens · 2024Article
- Bacterial DNA methyltransferase: A key to the epigenetic world with lessons learned from proteobacteria.Frontiers in microbiology · 2023Review
- Population analysis of Legionella pneumophila reveals a basis for resistance to complement-mediated killing.Nature communications · 2021Article
- The Role of Lipids inInternational journal of molecular sciences · 2021Review
- Phase Variation of LPS and Capsule Is Responsible for Stochastic Biofilm Formation inFrontiers in cellular and infection microbiology · 2021Article
- Temperature-Dependent Gene Expression inFrontiers in microbiology · 2018Article
- Active and adaptive Legionella CRISPR-Cas reveals a recurrent challenge to the pathogen.Cellular microbiology · 2016Article
- A new perspective on lysogeny: prophages as active regulatory switches of bacteria.Nature reviews. Microbiology · 2015Review
- Characterization of differential Toll-like receptor responses below the optical diffraction limit.Small (Weinheim an der Bergstrasse, Germany) · 2012Article
- Virulence properties of the legionella pneumophila cell envelope.Frontiers in microbiology · 2011Article
- The guinea pig as a model of infectious diseases.Comparative medicine · 2008Review
- Membrane vesicles shed by Legionella pneumophila inhibit fusion of phagosomes with lysosomes.Infection and immunity · 2006Article
- Phase and antigenic variation in bacteria.Clinical microbiology reviews · 2004Review
- Biochemical and functional analyses of the Mip protein: influence of the N-terminal half and of peptidylprolyl isomerase activity on the virulence of Legionella pneumophila.Infection and immunity · 2003Article
- A microbial strategy to multiply in macrophages: the pregnant pause.Traffic (Copenhagen, Denmark) · 2002Review
- Identification, characterization, and variation in expression of two serologically distinct O-antigen epitopes in lipopolysaccharides of Campylobacter fetus serotype A strains.Infection and immunity · 2001Article
- 3-Deoxy-D-manno-oct-2-ulosonic acid (Kdo) transferase of Legionella pneumophila transfers two kdo residues to a structurally different lipid A precursor of Escherichia coli.Journal of bacteriology · 2000Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the aid of monoclonal antibody (mAb) 2625, raised against the lipopolysaccharide (LPS) of Legionella pneumophila serogroup 1, subgroup OLDA, we isolated mutant 811 from the virulent wild-type strain RC1. This mutant was not reactive with mAb 2625 and exhibited an unstable phenotype, since we observed an in vitro and in vivo switch of mutant 811 to the mAb 2625-positive phenotype, thus restoring the wild-type LPS. Bactericidal assays revealed that mutant 811 was lysed by serum complement components, whereas the parental strain RC1 was almost serum resistant. Moreover, mutant 811 was not able to replicate intracellularly in macrophage-like cell line HL-60. In the guinea pig animal model, mutant 811 exhibited significantly reduced ability to replicate. Among recovered bacteria, mAb 2625-positive revertants were increased by fourfold. The relevance of LPS phase switch for pathogenesis of Legionella infection was further corroborated by the observation that 5% of the bacteria recovered from the lungs of guinea pigs infected with the wild-type strain RC1 were negative for mAb 2625 binding. These findings strongly indicate that under in vivo conditions switching between two LPS phenotypes occurs and may promote adaptation and replication of L. pneumophila. This is the first description of phase-variable expression of Legionella LPS.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.