ArticleJournal of clinical microbiology1998
Diagnostic use of PCR for detection of Pneumocystis carinii in oral wash samples.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 2 syntheses or guidelines pooled it, 111 citations in OpenAlex.
- Pooled it
- PCR diagnosis of Pneumocystis pneumonia: a bivariate meta-analysis.Journal of clinical microbiology · 2011Pooled it
- Review
- An overview of the laboratory diagnosis ofJournal of clinical microbiology · 2025Review
- Is It Possible to DifferentiateJournal of fungi (Basel, Switzerland) · 2021Article
- Diagnosing Pneumocystis jirovecii pneumonia: A review of current methods and novel approaches.Medical mycology · 2020Review
- Serum-based diagnosis of Pneumocystis pneumonia by detection of Pneumocystis jirovecii DNA and 1,3-β-D-glucan in HIV-infected patients: a retrospective case control study.BMC infectious diseases · 2019Article
- Comparative Evaluation Between the RealStarAnnals of laboratory medicine · 2019Article
- Detection of Pneumocystis jirovecii in oral wash from immunosuppressed patients as a diagnostic tool.PloS one · 2017Article
- Outbreak of pneumocystis pneumonia in renal and liver transplant patients caused by genotypically distinct strains of Pneumocystis jirovecii.Transplantation · 2013Article
- Use of nasopharyngeal aspirate for diagnosis of pneumocystis pneumonia.Journal of clinical microbiology · 2013Article
- Clinical characteristics of Pneumocystis pneumonia in non-HIV patients and prognostic factors including microbiological genotypes.BMC infectious diseases · 2011Article
- Update on the diagnosis and treatment of Pneumocystis pneumonia.Therapeutic advances in respiratory disease · 2011Review
- Article
- Infrequent detection of Pneumocystis jirovecii by PCR in oral wash specimens from TB patients with or without HIV and healthy contacts in Tanzania.BMC infectious diseases · 2010Article
- Principles and applications of polymerase chain reaction in medical diagnostic fields: a review.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2009Article
- Management of Pneumocystis jiroveci pneumonia in children receiving chemotherapy.Paediatric drugs · 2007Article
- Clinical applications of molecular biology for infectious diseases.The Clinical biochemist. Reviews · 2006Article
- Variations in emm type among group A streptococcal isolates causing invasive or noninvasive infections in a nationwide study.Journal of clinical microbiology · 2005Article
- Reemergence of emm1 and a changed superantigen profile for group A streptococci causing invasive infections: results from a nationwide study.Journal of clinical microbiology · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is a need to develop noninvasive methods for the diagnosis of Pneumocystis carinii pneumonia in patients unable to undergo bronchoscopy or induction sputum. Oral wash specimens are easily obtained, and P. carinii nucleic acid can be amplified and demonstrated by PCR. In routine clinical use, easy sample processing and single-round PCR are needed to ensure rapid analysis and to reduce the risk of contamination. We developed a single-round Touchdown PCR (TD-PCR) protocol with the ability to detect PCR inhibition in the specimen. The TD-PCR was evaluated in a routine diagnostic laboratory and was compared to a previously described PCR protocol (mitochondrial RNA) run in a research laboratory. Both PCR methods amplified a sequence of the mitochondrial rRNA gene of P. carinii. Paired bronchoalveolar lavage (BAL) and oral wash specimens from 76 consecutive human immunodeficiency virus type 1-infected persons undergoing a diagnostic bronchoscopy were included. The TD-PCR procedure was quicker than the mitochondrial PCR procedure (<24 versus 48 h) and, compared to microscopy, had sensitivity, specificity, and positive and negative predictive values of 89, 94, 93, and 91%, respectively, for oral wash specimens and 100, 91, 90, and 100%, respectively, for BAL specimens. Our results suggest that oral wash specimens are a potential noninvasive method to obtain a diagnostic specimen during P. carinii pneumonia infection and that it can be applied in a routine diagnostic laboratory.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.