ArticleGenetics1998
Autoregulation of transformer-2 alternative splicing is necessary for normal male fertility in Drosophila.
Article in Genetics, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- fmd/fmd2/DHX16-dsx cascade in sex determination of one Hemiptera species, Laodelphax striatellus.Insect science · 2026Article
- Male-biased Adult Production of the Striped Fruit Fly,Insects · 2020Article
- Auto-regulatory feedback by RNA-binding proteins.Journal of molecular cell biology · 2019Review
- The Nix locus on the male-specific homologue of chromosome 1 in Aedes albopictus is a strong candidate for a male-determining factor.Parasites & vectors · 2018Article
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- Article
- Altering the sex determination pathway in Drosophila fat body modifies sex-specific stress responses.American journal of physiology. Regulatory, integrative and comparative physiology · 2014Article
- Relationship between genetic polymorphisms in the HTR1A gene and paranoid schizophrenia in a northern Han Chinese population.Journal of molecular neuroscience : MN · 2013Article
- Article
- Regulated functional alternative splicing in Drosophila.Nucleic acids research · 2012Review
- Identification of evolutionarily conserved exons as regulated targets for the splicing activator tra2β in development.PLoS genetics · 2011Article
- The gene transformer-2 of Anastrepha fruit flies (Diptera, Tephritidae) and its evolution in insects.BMC evolutionary biology · 2010Article
- The doublesex splicing enhancer components Tra2 and Rbp1 also repress splicing through an intronic silencer.Molecular and cellular biology · 2007Article
- Concentration dependent selection of targets by an SR splicing regulator results in tissue-specific RNA processing.Nucleic acids research · 2006Article
- Direct repression of splicing by transformer-2.Molecular and cellular biology · 2003Article
- Autoregulation of FCA pre-mRNA processing controls Arabidopsis flowering time.The EMBO journal · 2003Article
- The Drosophila takeout gene is regulated by the somatic sex-determination pathway and affects male courtship behavior.Genes & development · 2002Article
- Functionally antagonistic sequences are required for normal autoregulation of Drosophila tra-2 pre-mRNA splicing.Nucleic acids research · 2001Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In the male germline of Drosophila the transformer-2 protein is required for differential splicing of pre-mRNAs from the exuperantia and att genes and autoregulates alternative splicing of its own pre-mRNA. Autoregulation of TRA-2 splicing results in production of two mRNAs that differ by the splicing/retention of the M1 intron and encode functionally distinct protein isoforms. Splicing of the intron produces an mRNA encoding TRA-2(226), which is necessary and sufficient for both male fertility and regulation of downstream target RNAs. When the intron is retained, an mRNA is produced encoding TRA-2(179), a protein with no known function. We have previously shown that repression of M1 splicing is dependent on TRA-2(226), suggesting that this protein quantitatively limits its own expression through a negative feedback mechanism at the level of splicing. Here we examine this idea, by testing the effect that variations in the level of tra-2 expression have on the splicing of M1 and on male fertility. Consistent with our hypothesis, we observe that as tra-2 gene dosage is increased, smaller proportions of TRA-2(226) mRNA are produced, limiting expression of this isoform. Feedback regulation is critical for male fertility, since it is significantly decreased by a transgene in which repression of M1 splicing cannot occur and TRA-2(226) mRNA is constitutively produced. The effect of this transgene becomes more severe as its dosage is increased, indicating that fertility is sensitive to an excess of TRA-2(226). Our results suggest that autoregulation of TRA-2(226) expression in male germ cells is necessary for normal spermatogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.