ArticleGenetics1998
Molecular screening for P-element insertions in a large genomic region of Drosophila melanogaster using polymerase chain reaction mediated by the vectorette.
Article in Genetics, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 24 citations in OpenAlex.
- Human transposon insertion profiling: Analysis, visualization and identification of somatic LINE-1 insertions in ovarian cancer.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Somatic retrotransposition is infrequent in glioblastomas.Mobile DNA · 2016Article
- The Chriz-Z4 complex recruits JIL-1 to polytene chromosomes, a requirement for interband-specific phosphorylation of H3S10.Journal of biosciences · 2011Article
- Article
- Transposon insertion site profiling chip (TIP-chip).Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Dissection of synaptic excitability phenotypes by using a dominant-negative Shaker K+ channel subunit.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The Drosophila kinesin-like protein KLP67A is essential for mitotic and male meiotic spindle assembly.Molecular biology of the cell · 2004Article
- Article
- Efficient recovery of centric heterochromatin P-element insertions in Drosophila melanogaster.Genetics · 2002Article
- Screening insertion libraries for mutations in many genes simultaneously using DNA microarrays.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As an alternative to existing methods for the detection of new insertions during a transposon mutagenesis, we adapted the method of vectorette ligation to genomic restriction fragments followed by PCR to obtain genomic sequences flanking the transposon. By combining flies containing a defined genomic transposon with an excess of flies containing unrelated insertion sites, we demonstrate the specificity and sensitivity of the procedure in the detection of integration events. This method was applied in a transposon-tagging screen for BJ1, the Drosophila homolog of the vertebrate gene Regulator of Chromosome Condensation (RCCI). Genetic mobilization of a single genomic P element was used to generate preferentially new local insertions from which integrations into a genomic region surrounding the BJ1 gene were screened. Flies harboring new insertions were phenotypically selected on the basis of the zeste1-dependent transvection of white. We detected a single transposition to a 13-kb region close to the BJ1 gene among 6650 progeny that were analyzed. Southern analysis of the homozygous line confirmed the integration 3 kb downstream of BJ1.
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