ArticleGenes & development1998
Self-renewal of pluripotent embryonic stem cells is mediated via activation of STAT3.
Article in Genes & development, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 585 papers, 2 of them syntheses that pooled it.
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Who cites it
585 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Dichotomous role of Shp2 for naïve and primed pluripotency maintenance in embryonic stem cells.Stem cell research & therapy · 2022Pooled it
- Prognostic Power of a Tumor Differentiation Gene Signature for Bladder Urothelial Carcinomas.Journal of the National Cancer Institute · 2018Pooled it
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- Conserved signaling pathways and transcriptional regulators drive ovarian aging across species.Poultry science · 2026Article
- Decoding the origins of cellular self-organization for engineered biology.Nature biotechnology · 2026Review
- Haploid Parthenotes Have Higher Demand for Glucose and Pyruvate Compared to Normally Fertilized Embryos During their In Vitro Development.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- Cancer-Associated STAT3 Mutations Maintain ES Cell Self-Renewal Through Phosphorylation-Independent Mechanisms.Genes to cells : devoted to molecular & cellular mechanisms · 2026Article
- Leukemia inhibitory factor drives malignant progression in lung squamous cell carcinoma via the PI3K/AKT/GSK-3β/β-catenin axis.Journal of molecular histology · 2026Article
- Nickel exposure disrupts epigenetic repression of developmental genes in mouse embryonic stem cells.Toxicology and applied pharmacology · 2026Article
- G3BP1 is a SWI/SNF-bound regulator of transcription that modulates activation of STATs.Bioscience reports · 2026Article
- Improving gene isoform quantification with miniQuant.Nature biotechnology · 2026Article
- Advances in porcine stem cell research and their applications in agriculture.Frontiers in cell and developmental biology · 2026Review
- Article
- Murine vascular organoids are responsive and adaptable 3D systems with cellular heterogeneity and dynamic plasticity.Science advances · 2025Article
- IPMK depletion influences genome-wide DNA methylation.Biochemical and biophysical research communications · 2025Article
- LIFR-Mediated ERBB2 Signaling Is Essential for Successful Embryo Implantation in Mice.Biomolecules · 2025Article
- The regulatory architecture of the primed pluripotent cell state.Nature communications · 2025Article
- Establishing Bovine Embryonic Stem Cells and Dissecting Their Self-Renewal Mechanisms.International journal of molecular sciences · 2025Article
- Crosstalk between Signaling Pathways and Energy Metabolism in Pluripotency.International journal of stem cells · 2025Review
- lncRNA NORAD modulates STAT3/STAT1 balance and innate immune responses in human cells via interaction with STAT3.Nature communications · 2025Article
525 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The propagation of embryonic stem (ES) cells in an undifferentiated pluripotent state is dependent on leukemia inhibitory factor (LIF) or related cytokines. These factors act through receptor complexes containing the signal transducer gp130. The downstream mechanisms that lead to ES cell self-renewal have not been delineated, however. In this study, chimeric receptors were introduced into ES cells. Biochemical and functional studies of transfected cells demonstrated a requirement for engagement and activation of the latent trancription factor STAT3. Detailed mutational analyses unexpectedly revealed that the four STAT3 docking sites in gp130 are not functionally equivalent. The role of STAT3 was then investigated using the dominant interfering mutant, STAT3F. ES cells that expressed this molecule constitutively could not be isolated. An episomal supertransfection strategy was therefore used to enable the consequences of STAT3F expression to be examined. In addition, an inducible STAT3F transgene was generated. In both cases, expression of STAT3F in ES cells growing in the presence of LIF specifically abrogated self-renewal and promoted differentiation. These complementary approaches establish that STAT3 plays a central role in the maintenance of the pluripotential stem cell phenotype. This contrasts with the involvement of STAT3 in the induction of differentiation in somatic cell types. Cell type-specific interpretation of STAT3 activation thus appears to be pivotal to the diverse developmental effects of the LIF family of cytokines. Identification of STAT3 as a key transcriptional determinant of ES cell self-renewal represents a first step in the molecular characterization of pluripotency.
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